|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
6
|
|
pubmed:dateCreated |
2002-5-8
|
|
pubmed:abstractText |
We determined the effect of aromatic amino acid stimulation of the human extracellular Ca2+-sensing receptor (CaR) on intracellular Ca2+ concentration ([Ca2+]i) in single HEK-293 cells. Addition of L-phenylalanine or L-tryptophan (at 5 mM) induced [Ca2+]i oscillations from a resting state that was quiescent at 1.8 mM extracellular Ca2+ concentration ([Ca2+]e). Each [Ca2+]i peak returned to baseline values, and the average oscillation frequency was approximately 1 min(-1) at 37 degrees C. Oscillations were not induced or sustained if the [Ca2+]e was reduced to 0.5 mM, even in the continued presence of amino acid. Average oscillation frequency in response to an increase in [Ca2+]e (from 1.8 to 2.5-5 mM) was much higher (approximately 4 min(-1)) than that induced by aromatic amino acids. Oscillations in response to [Ca2+]e were sinusoidal whereas those induced by amino acids were transient. Thus both amino acids and Ca2+, acting through the same CaR, produce oscillatory increases in [Ca2+]i, but the resultant oscillation pattern and frequency allow the cell to discriminate which agonist is bound to the receptor.
|
|
pubmed:grant |
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/2-aminoethoxydiphenyl borate,
http://linkedlifedata.com/resource/pubmed/chemical/Amino Acids,
http://linkedlifedata.com/resource/pubmed/chemical/Boron Compounds,
http://linkedlifedata.com/resource/pubmed/chemical/Calcium,
http://linkedlifedata.com/resource/pubmed/chemical/Calcium Channels,
http://linkedlifedata.com/resource/pubmed/chemical/Enzyme Inhibitors,
http://linkedlifedata.com/resource/pubmed/chemical/Fluorescent Dyes,
http://linkedlifedata.com/resource/pubmed/chemical/Fura-2,
http://linkedlifedata.com/resource/pubmed/chemical/ITPR1 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Inositol 1,4,5-Trisphosphate...,
http://linkedlifedata.com/resource/pubmed/chemical/Phenylalanine,
http://linkedlifedata.com/resource/pubmed/chemical/Protein Kinase C,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Calcium-Sensing,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Cell Surface,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Cytoplasmic and Nuclear,
http://linkedlifedata.com/resource/pubmed/chemical/Tryptophan,
http://linkedlifedata.com/resource/pubmed/chemical/fura-2-am
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Jun
|
|
pubmed:issn |
0363-6143
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:volume |
282
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
C1414-22
|
|
pubmed:dateRevised |
2007-11-15
|
|
pubmed:meshHeading |
pubmed-meshheading:11997256-Amino Acids,
pubmed-meshheading:11997256-Biological Clocks,
pubmed-meshheading:11997256-Boron Compounds,
pubmed-meshheading:11997256-Calcium,
pubmed-meshheading:11997256-Calcium Channels,
pubmed-meshheading:11997256-Calcium Signaling,
pubmed-meshheading:11997256-Cell Line,
pubmed-meshheading:11997256-Dose-Response Relationship, Drug,
pubmed-meshheading:11997256-Enzyme Inhibitors,
pubmed-meshheading:11997256-Fluorescent Dyes,
pubmed-meshheading:11997256-Fura-2,
pubmed-meshheading:11997256-Humans,
pubmed-meshheading:11997256-Inositol 1,4,5-Trisphosphate Receptors,
pubmed-meshheading:11997256-Intracellular Fluid,
pubmed-meshheading:11997256-Kidney,
pubmed-meshheading:11997256-Phenylalanine,
pubmed-meshheading:11997256-Protein Kinase C,
pubmed-meshheading:11997256-Receptors, Calcium-Sensing,
pubmed-meshheading:11997256-Receptors, Cell Surface,
pubmed-meshheading:11997256-Receptors, Cytoplasmic and Nuclear,
pubmed-meshheading:11997256-Transfection,
pubmed-meshheading:11997256-Tryptophan
|
|
pubmed:year |
2002
|
|
pubmed:articleTitle |
Amino acids and Ca2+ stimulate different patterns of Ca2+ oscillations through the Ca2+-sensing receptor.
|
|
pubmed:affiliation |
Unit of Signal Transduction and Gastrointestinal Cancer, Division of Digestive Diseases, Department of Medicine, School of Medicine, University of California, Los Angeles, California 90095-1786, USA.
|
|
pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
|
