Source:http://linkedlifedata.com/resource/pubmed/id/11995920
Switch to
Predicate | Object |
---|---|
rdf:type | |
lifeskim:mentions | |
pubmed:issue |
4
|
pubmed:dateCreated |
2002-5-8
|
pubmed:abstractText |
The effect of activins AB and B on DNA synthesis stimulated by epidermal growth factor (EGF) was studied in primary cultured rat hepatocytes and compared with the effect of activin A, a suppressor of DNA synthesis. Activin AB inhibited DNA synthesis as assessed by [3H]thymidine incorporation. The inhibition by activin AB was detected at 6 ng/ml, and the 12.5 ng/ml concentration produced almost maximal inhibition, approximately 40%, almost the same as that produced by activin A. Inhibition by activin A was detected at 3 ng/ml, and the 6 ng/ml concentration produced almost maximal inhibition. Activin B, on the other hand, had no effect on DNA synthesis up to 50 ng/ml. The increase in labeling index by EGF was also reduced to about 20% by 25 ng/ml activin A and activin AB, but not by activin B. Activin B, however, inhibited the binding of [125I]activin A to hepatocytes, but had no effect on the inhibition of DNA synthesis by activin A, even at 3-fold excess concentrations. These findings suggest that activin AB may act in the same manner as activin A does in terms EGF's inhibitory effect on DNA synthesis, although the effective concentration is higher than that of activin A. The findings also suggest that activin B receptors are present in hepatocytes but that they do not mediate signal transduction leading to the inhibition of DNA synthesis.
|
pubmed:language |
eng
|
pubmed:journal | |
pubmed:citationSubset |
IM
|
pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/DNA,
http://linkedlifedata.com/resource/pubmed/chemical/Epidermal Growth Factor,
http://linkedlifedata.com/resource/pubmed/chemical/Inhbb protein, rat,
http://linkedlifedata.com/resource/pubmed/chemical/Inhibin-beta Subunits,
http://linkedlifedata.com/resource/pubmed/chemical/inhibin beta A subunit
|
pubmed:status |
MEDLINE
|
pubmed:month |
Apr
|
pubmed:issn |
0918-6158
|
pubmed:author | |
pubmed:issnType |
Print
|
pubmed:volume |
25
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
437-40
|
pubmed:dateRevised |
2005-11-17
|
pubmed:meshHeading |
pubmed-meshheading:11995920-Animals,
pubmed-meshheading:11995920-Cells, Cultured,
pubmed-meshheading:11995920-DNA,
pubmed-meshheading:11995920-Epidermal Growth Factor,
pubmed-meshheading:11995920-Hepatocytes,
pubmed-meshheading:11995920-Inhibin-beta Subunits,
pubmed-meshheading:11995920-Male,
pubmed-meshheading:11995920-Rats,
pubmed-meshheading:11995920-Rats, Wistar
|
pubmed:year |
2002
|
pubmed:articleTitle |
Effect of activins AB and B on DNA synthesis stimulated by epidermal growth factor in primary cultured rat hepatocytes.
|
pubmed:affiliation |
Division of Biological Chemistry and Biologicals, National Institute of Health Sciences, Tokyo, Japan. niimi@nihs.go.jp
|
pubmed:publicationType |
Journal Article
|