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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
2002-5-23
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pubmed:abstractText |
Our aim was to examine the involvement of G(1) cell-cycle regulators in cell growth dysregulation induced by HTLV-I. Compared to uninfected cells, higher expression levels of cyclin D1 and D2 mRNA were detected in HTLV-I-infected T-cell lines, which were at least in part mediated by the viral transforming protein Tax since Tax activated both cyclin D1 and D2 promoters in the human T-cell line Jurkat. A Tax mutant that did not activate NF-kappaB failed to activate cyclin D1 and D2 promoters. Inhibitors of NF-kappaB (dominant negative IkappaBs mutants) suppressed Tax-dependent activation of cyclin D1 and D2 promoters, indicating that Tax-induced activation was mediated by NF-kappaB. Wild-type and mutant Tax capable of activating NF-kappaB, but not Tax mutant incapable of activating NF-kappaB, converted cell growth of a T-cell line from being IL-2-dependent to being IL-2-independent; and this conversion was associated with IL-2-independent induction of cyclins D1 and D2. Our data suggest that induction of cyclins D1 and D2 by Tax is involved in IL-2-independent cell-cycle progression as well as IL-2-independent transformation of primary human T cells by HTLV-I. High expression levels of cyclin D1 and D2 mRNAs were also detected in some patients with ATL. Our findings link HTLV-I infection to changes in cellular D-type cyclin gene expression, transformation of T cells and subsequent development of T-cell leukemia.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/CCND2 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Ccnd2 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclin D1,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclin D2,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclins,
http://linkedlifedata.com/resource/pubmed/chemical/Gene Products, tax,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-2,
http://linkedlifedata.com/resource/pubmed/chemical/NF-kappa B,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0020-7136
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pubmed:author |
pubmed-author:FujiiMasahiroM,
pubmed-author:HinzMichaelM,
pubmed-author:IkedaShuichiS,
pubmed-author:KashanchiFatahF,
pubmed-author:MoriNaokiN,
pubmed-author:NakayamaKatsushiK,
pubmed-author:TanakaYuetsuY,
pubmed-author:TomonagaMasaoM,
pubmed-author:YamadaYasuakiY,
pubmed-author:YamamotoNaokiN,
pubmed-author:YamasakiYoshihiroY
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pubmed:copyrightInfo |
Copyright 2002 Wiley-Liss, Inc.
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pubmed:issnType |
Print
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pubmed:day |
20
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pubmed:volume |
99
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
378-85
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:11992406-Animals,
pubmed-meshheading:11992406-Blotting, Northern,
pubmed-meshheading:11992406-Blotting, Western,
pubmed-meshheading:11992406-Cell Cycle,
pubmed-meshheading:11992406-Cell Division,
pubmed-meshheading:11992406-Cyclin D1,
pubmed-meshheading:11992406-Cyclin D2,
pubmed-meshheading:11992406-Cyclins,
pubmed-meshheading:11992406-Gene Products, tax,
pubmed-meshheading:11992406-Humans,
pubmed-meshheading:11992406-Interleukin-2,
pubmed-meshheading:11992406-Kinetics,
pubmed-meshheading:11992406-Leukemia, T-Cell,
pubmed-meshheading:11992406-Mice,
pubmed-meshheading:11992406-NF-kappa B,
pubmed-meshheading:11992406-Plasmids,
pubmed-meshheading:11992406-Promoter Regions, Genetic,
pubmed-meshheading:11992406-RNA, Messenger,
pubmed-meshheading:11992406-T-Lymphocytes,
pubmed-meshheading:11992406-Transcription, Genetic,
pubmed-meshheading:11992406-Transcriptional Activation,
pubmed-meshheading:11992406-Transfection,
pubmed-meshheading:11992406-Tumor Cells, Cultured
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pubmed:year |
2002
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pubmed:articleTitle |
Activation of cyclin D1 and D2 promoters by human T-cell leukemia virus type I tax protein is associated with IL-2-independent growth of T cells.
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pubmed:affiliation |
Department of Preventive Medicine and AIDS Research, Institute of Tropical Medicine, Nagasaki University, Nagasaki, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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