Source:http://linkedlifedata.com/resource/pubmed/id/11988512
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
Pt 5
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| pubmed:dateCreated |
2002-5-3
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| pubmed:databankReference | |
| pubmed:abstractText |
Recombination among bacterial extrachromosomal DNAs (EC-DNAs) plays a major evolutionary role by creating genetic diversity, and provides the potential for rapid adaptation to new environmental conditions. Previously, a 7 kbp EC-DNA, EcOYW1, with a geminivirus-like rolling-circle-replication protein (Rep) gene was isolated and characterized from an original wild-type line (OY-W) of onion yellows (OY) phytoplasma, an endocellular cell-wall-less prokaryote that inhabits the cytoplasm of both plant and insect cells. EcOYW1, found in OY-W, was not present in a mild-symptom line (OY-M) derived from OY-W. A 4 kbp EC-DNA, pOYW, was also isolated and characterized from OY-W, and its pLS1-plasmid-like rep gene was expressed. This paper describes the isolation and sequencing of an EC-DNA of 5560 nt, EcOYW2, from OY-W, and its counterpart EC-DNA of 5025 nt, EcOYM, from OY-M. EcOYW2 and EcOYM contained seven and six ORFs, respectively. They both encoded a geminivirus-like Rep and a putative single-stranded-DNA-binding protein (SSB). Southern blot analysis indicated that no more EC-DNAs with a rep gene exist in either OY-W or OY-M, which means that the complete set of EC-DNAs has been cloned from the OY-W and OY-M lines of OY phytoplasmas. Sequence analysis revealed that both EcOYW2 and EcOYM have chimeric structures of previously characterized EcOYW1 and pOYW, suggesting that they have a recombinational origin. This is the first evidence of intermolecular recombination between EC-DNAs in phytoplasma. The possible implications of these findings in increasing the biological diversity of phytoplasma are discussed.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Single-Stranded,
http://linkedlifedata.com/resource/pubmed/chemical/DNA Helicases,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Trans-Activators,
http://linkedlifedata.com/resource/pubmed/chemical/replication initiator protein
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| pubmed:status |
MEDLINE
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| pubmed:month |
May
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| pubmed:issn |
1350-0872
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:volume |
148
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
1389-96
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| pubmed:dateRevised |
2010-11-18
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| pubmed:meshHeading |
pubmed-meshheading:11988512-Amino Acid Sequence,
pubmed-meshheading:11988512-Blotting, Southern,
pubmed-meshheading:11988512-Cloning, Molecular,
pubmed-meshheading:11988512-DNA, Single-Stranded,
pubmed-meshheading:11988512-DNA Helicases,
pubmed-meshheading:11988512-DNA Replication,
pubmed-meshheading:11988512-DNA-Binding Proteins,
pubmed-meshheading:11988512-Genes, Bacterial,
pubmed-meshheading:11988512-Genetic Variation,
pubmed-meshheading:11988512-Molecular Sequence Data,
pubmed-meshheading:11988512-Open Reading Frames,
pubmed-meshheading:11988512-Plants,
pubmed-meshheading:11988512-Plasmids,
pubmed-meshheading:11988512-Recombination, Genetic,
pubmed-meshheading:11988512-Sequence Analysis, DNA,
pubmed-meshheading:11988512-Sequence Homology, Amino Acid,
pubmed-meshheading:11988512-Tenericutes,
pubmed-meshheading:11988512-Trans-Activators
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| pubmed:year |
2002
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| pubmed:articleTitle |
Evidence of intermolecular recombination between extrachromosomal DNAs in phytoplasma: a trigger for the biological diversity of phytoplasma?
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| pubmed:affiliation |
Graduate School of Agricultural and Life Sciences, The University of Tokyo, Kashiwa, Chiba 277-8562, Japan.
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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