Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2002-5-2
pubmed:abstractText
Gamma interferon (IFN-gamma) is an important mediator of endotoxin (lipopolysaccharide [LPS])-induced immune responses. However, the specific cell types that produce IFN-gamma in response to LPS and the cellular factors that regulate LPS-induced IFN-gamma production have not been fully determined. The present studies were undertaken to characterize the cell populations that produce IFN-gamma after LPS challenge in the spleens of mice and to determine the regulatory factors that modulate LPS-induced production of IFN-gamma. Our studies show that the levels of splenic IFN-gamma mRNA and protein production peak at 6 and 8 h, respectively, after systemic LPS challenge. Approximately 60% of IFN-gamma-producing cells are natural killer (NK) cells (CD3(-)DX5(+)) and 25% are NKT cells (CD3(+)DX5(+)). Most of the remaining IFN-gamma-producing cells are T cells (CD3(+)DX5(-)), macrophages, and dendritic cells. Functionally, interleukin-12 (IL-12) is the major IFN-gamma-stimulating factor after LPS challenge, with costimulation provided by IL-15, IL-18, and B7 proteins. IL-10 is a major inhibitor of LPS-induced IFN-gamma production. Unlike intact heat-killed gram-negative and gram-positive bacteria, the class II major histocompatibility complex did not play a functional role in LPS-induced IFN-gamma production. LPS is a potent stimulus for splenic IL-10, IL-12 p40, and IL-15 mRNA expression, whereas IL-12 p35 and IL-18 mRNAs, as well as B7 proteins, are constitutively expressed in the mouse spleen. Of the factors studied, IL-18 serves as the most potent costimulus with IL-12 for IFN-gamma production, followed by IL-15 and B7 proteins. These data demonstrate that NK cells and NKT cells are the most abundant IFN-gamma-producing cells in the mouse spleen after LPS challenge and that IL-10 and IL-12 are key functional regulators of LPS-induced IFN-gamma production.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/11986256-10047547, http://linkedlifedata.com/resource/pubmed/commentcorrection/11986256-10202018, http://linkedlifedata.com/resource/pubmed/commentcorrection/11986256-10227998, http://linkedlifedata.com/resource/pubmed/commentcorrection/11986256-10229825, http://linkedlifedata.com/resource/pubmed/commentcorrection/11986256-10377194, http://linkedlifedata.com/resource/pubmed/commentcorrection/11986256-10384100, http://linkedlifedata.com/resource/pubmed/commentcorrection/11986256-10510353, http://linkedlifedata.com/resource/pubmed/commentcorrection/11986256-10648125, http://linkedlifedata.com/resource/pubmed/commentcorrection/11986256-10657604, http://linkedlifedata.com/resource/pubmed/commentcorrection/11986256-10694580, http://linkedlifedata.com/resource/pubmed/commentcorrection/11986256-10706727, http://linkedlifedata.com/resource/pubmed/commentcorrection/11986256-10760800, http://linkedlifedata.com/resource/pubmed/commentcorrection/11986256-10820230, http://linkedlifedata.com/resource/pubmed/commentcorrection/11986256-10898491, http://linkedlifedata.com/resource/pubmed/commentcorrection/11986256-11034406, http://linkedlifedata.com/resource/pubmed/commentcorrection/11986256-11035061, http://linkedlifedata.com/resource/pubmed/commentcorrection/11986256-11093128, http://linkedlifedata.com/resource/pubmed/commentcorrection/11986256-11104806, http://linkedlifedata.com/resource/pubmed/commentcorrection/11986256-11207321, http://linkedlifedata.com/resource/pubmed/commentcorrection/11986256-11224518, http://linkedlifedata.com/resource/pubmed/commentcorrection/11986256-11244051, http://linkedlifedata.com/resource/pubmed/commentcorrection/11986256-11254700, http://linkedlifedata.com/resource/pubmed/commentcorrection/11986256-11342656, http://linkedlifedata.com/resource/pubmed/commentcorrection/11986256-11359846, http://linkedlifedata.com/resource/pubmed/commentcorrection/11986256-11378475, http://linkedlifedata.com/resource/pubmed/commentcorrection/11986256-11390504, http://linkedlifedata.com/resource/pubmed/commentcorrection/11986256-11500393, http://linkedlifedata.com/resource/pubmed/commentcorrection/11986256-1303622, http://linkedlifedata.com/resource/pubmed/commentcorrection/11986256-7760015, http://linkedlifedata.com/resource/pubmed/commentcorrection/11986256-8543827, http://linkedlifedata.com/resource/pubmed/commentcorrection/11986256-8675621, http://linkedlifedata.com/resource/pubmed/commentcorrection/11986256-9143706, http://linkedlifedata.com/resource/pubmed/commentcorrection/11986256-9176497, http://linkedlifedata.com/resource/pubmed/commentcorrection/11986256-9531301, http://linkedlifedata.com/resource/pubmed/commentcorrection/11986256-9531314, http://linkedlifedata.com/resource/pubmed/commentcorrection/11986256-9597139, http://linkedlifedata.com/resource/pubmed/commentcorrection/11986256-9625771
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
1071-412X
pubmed:author
pubmed:issnType
Print
pubmed:volume
9
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
530-43
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed-meshheading:11986256-Animals, pubmed-meshheading:11986256-Antigens, CD28, pubmed-meshheading:11986256-Antigens, CD80, pubmed-meshheading:11986256-Cells, Cultured, pubmed-meshheading:11986256-Dendritic Cells, pubmed-meshheading:11986256-Female, pubmed-meshheading:11986256-Gene Expression Profiling, pubmed-meshheading:11986256-Histocompatibility Antigens Class II, pubmed-meshheading:11986256-Interferon-gamma, pubmed-meshheading:11986256-Interleukin-12, pubmed-meshheading:11986256-Interleukin-15, pubmed-meshheading:11986256-Interleukin-18, pubmed-meshheading:11986256-Killer Cells, Natural, pubmed-meshheading:11986256-Lipopolysaccharides, pubmed-meshheading:11986256-Macrophages, Peritoneal, pubmed-meshheading:11986256-Mice, pubmed-meshheading:11986256-Mice, Inbred BALB C, pubmed-meshheading:11986256-RNA, Messenger, pubmed-meshheading:11986256-Signal Transduction, pubmed-meshheading:11986256-Spleen, pubmed-meshheading:11986256-T-Lymphocytes
pubmed:year
2002
pubmed:articleTitle
Endotoxin-induced gamma interferon production: contributing cell types and key regulatory factors.
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