| pubmed-article:11982500 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:11982500 | lifeskim:mentions | umls-concept:C0010453 | lld:lifeskim |
| pubmed-article:11982500 | lifeskim:mentions | umls-concept:C0034721 | lld:lifeskim |
| pubmed-article:11982500 | lifeskim:mentions | umls-concept:C0034693 | lld:lifeskim |
| pubmed-article:11982500 | lifeskim:mentions | umls-concept:C0225698 | lld:lifeskim |
| pubmed-article:11982500 | lifeskim:mentions | umls-concept:C0017742 | lld:lifeskim |
| pubmed-article:11982500 | lifeskim:mentions | umls-concept:C0205409 | lld:lifeskim |
| pubmed-article:11982500 | lifeskim:mentions | umls-concept:C0017262 | lld:lifeskim |
| pubmed-article:11982500 | pubmed:issue | 1 | lld:pubmed |
| pubmed-article:11982500 | pubmed:dateCreated | 2002-5-1 | lld:pubmed |
| pubmed-article:11982500 | pubmed:abstractText | Alveolar epithelium in situ takes up luminal glucose by cotransport with sodium. Cultured alveolar type II pneumocytes have only sodium-independent glucose uptake. It is unclear which isoforms are responsible for glucose transport in these cells and why sodium-glucose cotransport activity disappears during culture. GLUT1, GLUT4, GLUT5 and SGLT1 mRNA were detected in freshly isolated rat alveolar type II cells by reverse transcriptase-polymerase chain reaction. We show that SGLT1 mRNA was 90% lower in cells cultured in plastic wells for 2 or 4 days than in freshly isolated cells. mRNAs coding for the facilitated transporters were reduced from 40% (GLUT1) and 75% (GLUT4 and GLUT5) in cultured cells. Cells cultured at the air-liquid interface better preserved their phenotype as attested by significantly higher surfactant-associated protein mRNA levels. However, these cells had no higher GLUT1 and SGLT1 gene expression. Thus, alveolar type II cells lose sodium-glucose cotransport activity in part because of a decrease in mRNA levels. These changes in gene expression and/or mRNA stability may be an additional consequence of the shift towards the type I cell phenotype observed in cultured type II pneumocytes. | lld:pubmed |
| pubmed-article:11982500 | pubmed:language | eng | lld:pubmed |
| pubmed-article:11982500 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11982500 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:11982500 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11982500 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11982500 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11982500 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11982500 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11982500 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11982500 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11982500 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11982500 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11982500 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11982500 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11982500 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11982500 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:11982500 | pubmed:month | May | lld:pubmed |
| pubmed-article:11982500 | pubmed:issn | 0001-6772 | lld:pubmed |
| pubmed-article:11982500 | pubmed:author | pubmed-author:SaumonGG | lld:pubmed |
| pubmed-article:11982500 | pubmed:author | pubmed-author:MamchaouiKK | lld:pubmed |
| pubmed-article:11982500 | pubmed:author | pubmed-author:MakhloufiYY | lld:pubmed |
| pubmed-article:11982500 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:11982500 | pubmed:volume | 175 | lld:pubmed |
| pubmed-article:11982500 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:11982500 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:11982500 | pubmed:pagination | 19-24 | lld:pubmed |
| pubmed-article:11982500 | pubmed:dateRevised | 2005-11-17 | lld:pubmed |
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| pubmed-article:11982500 | pubmed:meshHeading | pubmed-meshheading:11982500... | lld:pubmed |
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| pubmed-article:11982500 | pubmed:meshHeading | pubmed-meshheading:11982500... | lld:pubmed |
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| pubmed-article:11982500 | pubmed:meshHeading | pubmed-meshheading:11982500... | lld:pubmed |
| pubmed-article:11982500 | pubmed:meshHeading | pubmed-meshheading:11982500... | lld:pubmed |
| pubmed-article:11982500 | pubmed:meshHeading | pubmed-meshheading:11982500... | lld:pubmed |
| pubmed-article:11982500 | pubmed:year | 2002 | lld:pubmed |
| pubmed-article:11982500 | pubmed:articleTitle | Glucose transporter gene expression in freshly isolated and cultured rat pneumocytes. | lld:pubmed |
| pubmed-article:11982500 | pubmed:affiliation | INSERM U82, Faculté Xavier Bichat, Paris, France. | lld:pubmed |
| pubmed-article:11982500 | pubmed:publicationType | Journal Article | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:11982500 | lld:pubmed |
