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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
2002-5-1
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pubmed:abstractText |
Alveolar epithelium in situ takes up luminal glucose by cotransport with sodium. Cultured alveolar type II pneumocytes have only sodium-independent glucose uptake. It is unclear which isoforms are responsible for glucose transport in these cells and why sodium-glucose cotransport activity disappears during culture. GLUT1, GLUT4, GLUT5 and SGLT1 mRNA were detected in freshly isolated rat alveolar type II cells by reverse transcriptase-polymerase chain reaction. We show that SGLT1 mRNA was 90% lower in cells cultured in plastic wells for 2 or 4 days than in freshly isolated cells. mRNAs coding for the facilitated transporters were reduced from 40% (GLUT1) and 75% (GLUT4 and GLUT5) in cultured cells. Cells cultured at the air-liquid interface better preserved their phenotype as attested by significantly higher surfactant-associated protein mRNA levels. However, these cells had no higher GLUT1 and SGLT1 gene expression. Thus, alveolar type II cells lose sodium-glucose cotransport activity in part because of a decrease in mRNA levels. These changes in gene expression and/or mRNA stability may be an additional consequence of the shift towards the type I cell phenotype observed in cultured type II pneumocytes.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Glucose,
http://linkedlifedata.com/resource/pubmed/chemical/Glucose Transporter Type 1,
http://linkedlifedata.com/resource/pubmed/chemical/Glucose Transporter Type 4,
http://linkedlifedata.com/resource/pubmed/chemical/Glucose Transporter Type 5,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Glycoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/Monosaccharide Transport Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Muscle Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Slc2a1 protein, rat,
http://linkedlifedata.com/resource/pubmed/chemical/Slc2a4 protein, rat,
http://linkedlifedata.com/resource/pubmed/chemical/Slc5a1 protein, rat,
http://linkedlifedata.com/resource/pubmed/chemical/Sodium,
http://linkedlifedata.com/resource/pubmed/chemical/Sodium-Glucose Transporter 1
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0001-6772
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
175
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
19-24
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pubmed:dateRevised |
2005-11-17
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pubmed:meshHeading |
pubmed-meshheading:11982500-Animals,
pubmed-meshheading:11982500-Cell Differentiation,
pubmed-meshheading:11982500-Cells, Cultured,
pubmed-meshheading:11982500-Gene Expression,
pubmed-meshheading:11982500-Glucose,
pubmed-meshheading:11982500-Glucose Transporter Type 1,
pubmed-meshheading:11982500-Glucose Transporter Type 4,
pubmed-meshheading:11982500-Glucose Transporter Type 5,
pubmed-meshheading:11982500-Male,
pubmed-meshheading:11982500-Membrane Glycoproteins,
pubmed-meshheading:11982500-Monosaccharide Transport Proteins,
pubmed-meshheading:11982500-Muscle Proteins,
pubmed-meshheading:11982500-Phenotype,
pubmed-meshheading:11982500-Pulmonary Alveoli,
pubmed-meshheading:11982500-Rats,
pubmed-meshheading:11982500-Rats, Sprague-Dawley,
pubmed-meshheading:11982500-Respiratory Mucosa,
pubmed-meshheading:11982500-Sodium,
pubmed-meshheading:11982500-Sodium-Glucose Transporter 1
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pubmed:year |
2002
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pubmed:articleTitle |
Glucose transporter gene expression in freshly isolated and cultured rat pneumocytes.
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pubmed:affiliation |
INSERM U82, Faculté Xavier Bichat, Paris, France.
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pubmed:publicationType |
Journal Article
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