Linked Life Data

11980944

Source:http://linkedlifedata.com/resource/pubmed/id/11980944

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pubmed-article:11980944pubmed:abstractTextA commercially prepared frozen broth microdilution panel (Trek Diagnostic Systems, Westlake, Ohio) was compared with a reference microdilution panel for antifungal susceptibility testing of two quality control (QC) strains and 99 clinical isolates of Candida spp. The antifungal agents tested included amphotericin B, flucytosine, fluconazole, itraconazole, posaconazole, ravuconazole, and voriconazole. Microdilution testing was performed according to NCCLS recommendations. MIC endpoints were read visually after 48 h of incubation and were assessed independently for each microdilution panel. The MICs for the QC strains were within published limits for both the reference and Trek microdilution panels. Discrepancies among MIC endpoints of no more than 2 dilutions were used to calculate the percent agreement. Acceptable levels of agreement between the Trek and reference panels were observed for all antifungal agents tested against the 99 clinical isolates. The overall agreement for each antifungal agent ranged from 96% for ravuconazole to 100% for amphotericin B. The Trek microdilution panel appears to be a viable alternative to frozen microdilution panels prepared in-house.lld:pubmed
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pubmed-article:11980944pubmed:monthMaylld:pubmed
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pubmed-article:11980944pubmed:authorpubmed-author:HuynhHHlld:pubmed
pubmed-article:11980944pubmed:authorpubmed-author:PfallerM AMAlld:pubmed
pubmed-article:11980944pubmed:authorpubmed-author:HollisR JRJlld:pubmed
pubmed-article:11980944pubmed:authorpubmed-author:DiekemaD JDJlld:pubmed
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pubmed-article:11980944pubmed:authorpubmed-author:MesserS ASAlld:pubmed
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pubmed-article:11980944pubmed:volume40lld:pubmed
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pubmed-article:11980944pubmed:pagination1694-7lld:pubmed
pubmed-article:11980944pubmed:dateRevised2009-11-18lld:pubmed
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pubmed-article:11980944pubmed:articleTitleClinical evaluation of a frozen commercially prepared microdilution panel for antifungal susceptibility testing of seven antifungal agents, including the new triazoles posaconazole, ravuconazole, and voriconazole.lld:pubmed
pubmed-article:11980944pubmed:affiliationMedical Microbiology Division, C606 GH, Department of Pathology, University of Iowa College of Medicine, Iowa City, IA 52242, USA. michael-pfaller@uiowa.edulld:pubmed
pubmed-article:11980944pubmed:publicationTypeJournal Articlelld:pubmed
pubmed-article:11980944pubmed:publicationTypeComparative Studylld:pubmed
pubmed-article:11980944pubmed:publicationTypeResearch Support, Non-U.S. Gov'tlld:pubmed
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