Source:http://linkedlifedata.com/resource/pubmed/id/11980641
Switch to
Predicate | Object |
---|---|
rdf:type | |
lifeskim:mentions | |
pubmed:issue |
9
|
pubmed:dateCreated |
2002-4-30
|
pubmed:abstractText |
Neural Wiskott-Aldrich syndrome protein (N-WASP), a member of the WASP family, regulates reorganization of the actin cytoskeleton through activation of the Arp2/3 complex. To date, most studies of N-WASP have focused on intracellular and morphological phenomena, such as vesicle transport and filopodium formation. We investigated the importance of N-WASP in epithelial morphogenesis, using Madin-Darby canine kidney epithelial cells, which form branching tubules when cultured with hepatocyte growth factor (HGF) in collagen gel. We established MDCK cell lines that overexpress wild-type N-WASP (WT-NW) or a dominant-negative form of N-WASP (DN-NW). WT-NW and parental Madin-Darby canine kidney cells formed branching tubules in collagen gel in response to HGF. However, formation of branching tubules was suppressed in DN-NW cells. During tubulogenesis, endogenous N-WASP accumulated at cell extensions protruding from the walls of the cysts and at the tips of the extending tubules. Gross cell morphology, cell-cell adhesion, cell polarity, and scattering in response to HGF were unaffected in WT-NW and DN-NW cells. In contrast, directed cell migration and HGF-induced invasion were significantly repressed in DN-NW cells. These results indicate that N-WASP regulates HGF-induced cell migration and invasion, which are required for epithelial tubulogenesis.
|
pubmed:language |
eng
|
pubmed:journal | |
pubmed:citationSubset |
IM
|
pubmed:chemical | |
pubmed:status |
MEDLINE
|
pubmed:month |
May
|
pubmed:issn |
0008-5472
|
pubmed:author | |
pubmed:issnType |
Print
|
pubmed:day |
1
|
pubmed:volume |
62
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
2503-9
|
pubmed:dateRevised |
2006-11-15
|
pubmed:meshHeading |
pubmed-meshheading:11980641-Animals,
pubmed-meshheading:11980641-Cell Line,
pubmed-meshheading:11980641-Cell Movement,
pubmed-meshheading:11980641-Dogs,
pubmed-meshheading:11980641-Epithelial Cells,
pubmed-meshheading:11980641-Hepatocyte Growth Factor,
pubmed-meshheading:11980641-Kidney Tubules,
pubmed-meshheading:11980641-Nerve Tissue Proteins,
pubmed-meshheading:11980641-Wiskott-Aldrich Syndrome Protein, Neuronal
|
pubmed:year |
2002
|
pubmed:articleTitle |
Neural Wiskott-Aldrich syndrome protein is involved in hepatocyte growth factor-induced migration, invasion, and tubulogenesis of epithelial cells.
|
pubmed:affiliation |
Division of Biochemistry, Institute of Medical Science, University of Tokyo, Tokyo 108-8639, Japan.
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|