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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
9
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pubmed:dateCreated |
2002-4-29
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pubmed:abstractText |
Transforming growth factor beta1 (TGF-beta1) induces long-term synaptic facilitation and long-term increases in excitability in Aplysia. Here we report that this growth factor has acute effects as well. Treatment of pleural-pedal ganglia with TGF-beta1 for 5 min activated mitogen-activated protein kinase (MAPK) and stimulated the phosphorylation of synapsin in a MAPK-dependent manner. This phosphorylation appeared to modulate synapsin distribution in cultured sensory neurons. Control neurons exhibited a punctate distribution of synapsin along neurites, which appeared to represent high concentration aggregates of synapsin. TGF-beta1-treated sensory neurons showed a significant reduction in the number of these puncta, an effect that was blocked by the MAP/ERK kinase inhibitor U0126. The functional consequence of TGF-beta1 was tested by examining its effects on synaptic transmission at the sensorimotor synapse. Application of TGF-beta1 reduced the magnitude of synaptic depression. This effect was dependent on MAPK, consistent with the hypothesis that TGF-1 mobilizes synaptic vesicles through the phosphorylation of synapsin.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
1529-2401
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:day |
1
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pubmed:volume |
22
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
RC220
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:11978861-Animals,
pubmed-meshheading:11978861-Aplysia,
pubmed-meshheading:11978861-Butadienes,
pubmed-meshheading:11978861-Cells, Cultured,
pubmed-meshheading:11978861-Electric Stimulation,
pubmed-meshheading:11978861-Enzyme Activation,
pubmed-meshheading:11978861-Enzyme Inhibitors,
pubmed-meshheading:11978861-Excitatory Postsynaptic Potentials,
pubmed-meshheading:11978861-Ganglia, Invertebrate,
pubmed-meshheading:11978861-Macromolecular Substances,
pubmed-meshheading:11978861-Mitogen-Activated Protein Kinases,
pubmed-meshheading:11978861-Motor Neurons,
pubmed-meshheading:11978861-Neural Inhibition,
pubmed-meshheading:11978861-Neurites,
pubmed-meshheading:11978861-Neurons, Afferent,
pubmed-meshheading:11978861-Nitriles,
pubmed-meshheading:11978861-Phosphorylation,
pubmed-meshheading:11978861-Synapses,
pubmed-meshheading:11978861-Synapsins,
pubmed-meshheading:11978861-Synaptic Transmission,
pubmed-meshheading:11978861-Synaptic Vesicles,
pubmed-meshheading:11978861-Transforming Growth Factor beta,
pubmed-meshheading:11978861-Transforming Growth Factor beta1
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pubmed:year |
2002
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pubmed:articleTitle |
Transforming growth factor beta1 alters synapsin distribution and modulates synaptic depression in Aplysia.
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pubmed:affiliation |
Department of Neurobiology and Anatomy, W. M. Keck Center for the Neurobiology of Learning and Memory, University of Texas-Houston Medical School, Houston, Texas 77030, USA.
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pubmed:publicationType |
Journal Article,
In Vitro,
Research Support, U.S. Gov't, P.H.S.
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