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rdf:type |
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lifeskim:mentions |
umls-concept:C0030685,
umls-concept:C0033371,
umls-concept:C0035820,
umls-concept:C0204727,
umls-concept:C0205409,
umls-concept:C0391871,
umls-concept:C0439834,
umls-concept:C0521116,
umls-concept:C0680255,
umls-concept:C1283071,
umls-concept:C1517714,
umls-concept:C1963578
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pubmed:issue |
9
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pubmed:dateCreated |
2002-4-29
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pubmed:abstractText |
Native rat lactotrophs express thyrotrophin-releasing hormone-dependent K+ currents consisting of fast and slow deactivating components that are both sensitive to the class III anti-arrhythmic drugs that block the eag-related gene (ERG) K+ current (I(ERG)). Here we describe in MMQ prolactin-releasing pituitary cells the isolation of the slowly deactivating long-lasting component (I(ERGS)), which, unlike the fast component (I(ERGF)), is insensitive to verapamil 2 microm but sensitive to a novel scorpion toxin (ErgTx-2) that hardly affects I(ERGF). The time constants of I(ERGS) activation, deactivation, and recovery from inactivation are more than one order of magnitude greater than in I(ERGF), and the voltage-dependent inactivation is left-shifted by approximately 25 mV. The very slow MMQ firing frequency (approximately 0.2 Hz) investigated in perforated patch is increased approximately four times by anti-arrhythmic agents, by ErgTx-2, and by the abrupt I(ERGS) deactivation. Prolactin secretion in the presence of anti-arrhythmics is three- to fourfold higher in comparison with controls. We provide evidence from I(ERGS) and I(ERGF) simulations in a firing model cell to indicate that only I(ERGS) has an accommodating role during the experimentally observed very slow firing. Thus, we suggest that I(ERGS) potently modulates both firing and prolactin release in lactotroph cells.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Anti-Arrhythmia Agents,
http://linkedlifedata.com/resource/pubmed/chemical/Calcium Channel Blockers,
http://linkedlifedata.com/resource/pubmed/chemical/Cation Transport Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/ERG1 potassium channel,
http://linkedlifedata.com/resource/pubmed/chemical/Erg2 protein, rat,
http://linkedlifedata.com/resource/pubmed/chemical/Ether-A-Go-Go Potassium Channels,
http://linkedlifedata.com/resource/pubmed/chemical/KCNH6 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/KCNH7 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Kcnh7 protein, rat,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Transport Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Potassium,
http://linkedlifedata.com/resource/pubmed/chemical/Potassium Channels,
http://linkedlifedata.com/resource/pubmed/chemical/Potassium Channels, Voltage-Gated,
http://linkedlifedata.com/resource/pubmed/chemical/Prolactin,
http://linkedlifedata.com/resource/pubmed/chemical/RNA,
http://linkedlifedata.com/resource/pubmed/chemical/Scorpion Venoms,
http://linkedlifedata.com/resource/pubmed/chemical/Tretinoin,
http://linkedlifedata.com/resource/pubmed/chemical/Verapamil
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
1529-2401
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pubmed:author |
pubmed-author:ArcangeliAnnarosaA,
pubmed-author:CassuliniRita RestanoRR,
pubmed-author:CrocianiOliviaO,
pubmed-author:CuriaGiuliaG,
pubmed-author:FlorioTullioT,
pubmed-author:GurrolaGeorginaG,
pubmed-author:LecchiMarziaM,
pubmed-author:MasiAlessioA,
pubmed-author:OlivottoMassimoM,
pubmed-author:PossaniLourival DLD,
pubmed-author:RedaelliElisaE,
pubmed-author:RosatiBarbaraB,
pubmed-author:SchettiniGennaroG,
pubmed-author:WankeEnzoE
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pubmed:issnType |
Electronic
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pubmed:day |
1
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pubmed:volume |
22
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
3414-25
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pubmed:dateRevised |
2008-10-28
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pubmed:meshHeading |
pubmed-meshheading:11978818-Action Potentials,
pubmed-meshheading:11978818-Animals,
pubmed-meshheading:11978818-Anti-Arrhythmia Agents,
pubmed-meshheading:11978818-Brain,
pubmed-meshheading:11978818-Calcium Channel Blockers,
pubmed-meshheading:11978818-Cation Transport Proteins,
pubmed-meshheading:11978818-Cell Line,
pubmed-meshheading:11978818-Computer Simulation,
pubmed-meshheading:11978818-Dose-Response Relationship, Drug,
pubmed-meshheading:11978818-Ether-A-Go-Go Potassium Channels,
pubmed-meshheading:11978818-Membrane Transport Proteins,
pubmed-meshheading:11978818-Models, Neurological,
pubmed-meshheading:11978818-Patch-Clamp Techniques,
pubmed-meshheading:11978818-Pituitary Gland,
pubmed-meshheading:11978818-Potassium,
pubmed-meshheading:11978818-Potassium Channels,
pubmed-meshheading:11978818-Potassium Channels, Voltage-Gated,
pubmed-meshheading:11978818-Prolactin,
pubmed-meshheading:11978818-RNA,
pubmed-meshheading:11978818-Rats,
pubmed-meshheading:11978818-Scorpion Venoms,
pubmed-meshheading:11978818-Tretinoin,
pubmed-meshheading:11978818-Verapamil
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pubmed:year |
2002
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pubmed:articleTitle |
Isolation of a long-lasting eag-related gene-type K+ current in MMQ lactotrophs and its accommodating role during slow firing and prolactin release.
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pubmed:affiliation |
Department of Biotechnology and Biosciences, University of Milano-Bicocca, I-20126 Milano, Italy.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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