11976100

Source:http://linkedlifedata.com/resource/pubmed/id/11976100

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Predicate	Object
rdf:type	pubmed:Citation
lifeskim:mentions	umls-concept:C0030015, umls-concept:C0301630, umls-concept:C0441712, umls-concept:C1011587, umls-concept:C2346593
pubmed:issue	5
pubmed:dateCreated	2002-4-26
pubmed:abstractText	Mechanisms for Fe(III) oxide reduction were investigated in Geothrix fermentans, a dissimilatory Fe(III)-reducing microorganism found within the Fe(III) reduction zone of subsurface environments. Culture filtrates of G. fermentans stimulated the reduction of poorly crystalline Fe(III) oxide by washed cell suspensions, suggesting that G. fermentans released one or more extracellular compounds that promoted Fe(III) oxide reduction. In order to determine if G. fermentans released electron-shuttling compounds, poorly crystalline Fe(III) oxide was incorporated into microporous alginate beads, which prevented contact between G. fermentans and the Fe(III) oxide. G. fermentans reduced the Fe(III) within the beads, suggesting that one of the compounds that G. fermentans releases is an electron-shuttling compound that can transfer electrons from the cell to Fe(III) oxide that is not in contact with the organism. Analysis of culture filtrates by thin-layer chromatography suggested that the electron shuttle has characteristics similar to those of a water-soluble quinone. Analysis of filtrates by ion chromatography demonstrated that there was as much as 250 microM dissolved Fe(III) in cultures of G. fermentans growing with Fe(III) oxide as the electron acceptor, suggesting that G. fermentans released one or more compounds capable of chelating and solubilizing Fe(III). Solubilizing Fe(III) is another strategy for alleviating the need for contact between cells and Fe(III) oxide for Fe(III) reduction. This is the first demonstration of a microorganism that, in defined medium without added electron shuttles or chelators, can reduce Fe(III) derived from Fe(III) oxide without directly contacting the Fe(III) oxide. These results are in marked contrast to those with Geobacter metallireducens, which does not produce electron shuttles or Fe(III) chelators. These results demonstrate that phylogenetically distinct Fe(III)-reducing microorganisms may use significantly different strategies for Fe(III) reduction. Thus, it is important to know which Fe(III)-reducing microorganisms predominate in a given environment in order to understand the mechanisms for Fe(III) reduction in the environment of interest.
pubmed:commentsCorrections	http://linkedlifedata.com/resource/pubmed/commentcorrection/11976100-10388703, http://linkedlifedata.com/resource/pubmed/commentcorrection/11976100-10555343, http://linkedlifedata.com/resource/pubmed/commentcorrection/11976100-10601229, http://linkedlifedata.com/resource/pubmed/commentcorrection/11976100-10788411, http://linkedlifedata.com/resource/pubmed/commentcorrection/11976100-11179660, http://linkedlifedata.com/resource/pubmed/commentcorrection/11976100-11225719, http://linkedlifedata.com/resource/pubmed/commentcorrection/11976100-11355193, http://linkedlifedata.com/resource/pubmed/commentcorrection/11976100-1886521, http://linkedlifedata.com/resource/pubmed/commentcorrection/11976100-390357, http://linkedlifedata.com/resource/pubmed/commentcorrection/11976100-4598231, http://linkedlifedata.com/resource/pubmed/commentcorrection/11976100-8022480, http://linkedlifedata.com/resource/pubmed/commentcorrection/11976100-8387263, http://linkedlifedata.com/resource/pubmed/commentcorrection/11976100-8636045, http://linkedlifedata.com/resource/pubmed/commentcorrection/11976100-9658015, http://linkedlifedata.com/resource/pubmed/commentcorrection/11976100-9687484
pubmed:language	eng
pubmed:journal	http://linkedlifedata.com/resource/pubmed/journal/7605801
pubmed:citationSubset	IM
pubmed:chemical	http://linkedlifedata.com/resource/pubmed/chemical/Ferric Compounds, http://linkedlifedata.com/resource/pubmed/chemical/Iron Chelating Agents, http://linkedlifedata.com/resource/pubmed/chemical/ferric oxide
pubmed:status	MEDLINE
pubmed:month	May
pubmed:issn	0099-2240
pubmed:author	pubmed-author:LovleyDerek RDR, pubmed-author:NevinKelly PKP
pubmed:issnType	Print
pubmed:volume	68
pubmed:owner	NLM
pubmed:authorsComplete	Y
pubmed:pagination	2294-9
pubmed:dateRevised	2010-9-14
pubmed:meshHeading	pubmed-meshheading:11976100-Bacteria, pubmed-meshheading:11976100-Electrons, pubmed-meshheading:11976100-Ferric Compounds, pubmed-meshheading:11976100-Iron Chelating Agents, pubmed-meshheading:11976100-Oxidation-Reduction, pubmed-meshheading:11976100-Solubility
pubmed:year	2002
pubmed:articleTitle	Mechanisms for accessing insoluble Fe(III) oxide during dissimilatory Fe(III) reduction by Geothrix fermentans.
pubmed:affiliation	Department of Microbiology, University of Massachusetts, Amherst, Massachusetts 01003, USA.
pubmed:publicationType	Journal Article, Research Support, U.S. Gov't, Non-P.H.S., Research Support, Non-U.S. Gov't