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rdf:type |
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lifeskim:mentions |
umls-concept:C0001483,
umls-concept:C0010825,
umls-concept:C0011306,
umls-concept:C0014644,
umls-concept:C0021083,
umls-concept:C0033684,
umls-concept:C0039195,
umls-concept:C0205263,
umls-concept:C0205314,
umls-concept:C0449445,
umls-concept:C0679622,
umls-concept:C0871261,
umls-concept:C1704632,
umls-concept:C1706817,
umls-concept:C2911692
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pubmed:issue |
7
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pubmed:dateCreated |
2002-4-26
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pubmed:abstractText |
Cytomegalovirus (CMV), Epstein-Barr virus (EBV), and adenovirus (Ad) cause significant morbidity and mortality in immunocompromised patients undergoing allogeneic stem cell transplantation. We have established a procedure to generate polyclonal cytotoxic T lymphocyte (CTL) populations with specificity against Ad and CMV or against Ad and EBV. Healthy donor-derived dendritic cells (DCs) were transduced with recombinant adenovirus encoding either CMV pp65 or EBV EBNA3C and used to stimulate autologous T cells. Stimulated T lymphocytes displayed specific simultaneous cytotoxicity against CMV and adenovirus and to a lesser extent against adenovirus and EBV. Recombinant vaccinia virus encoding individual adenovirus proteins showed that the T cell response to the adenovirus was directed mainly against the capsid protein hexon. The frequency of IFN-gamma-secreting T cells was 0.02% for adenovirus alone, and 0.05 and 0.14% for adenoviruses encoding EBNA3C and pp65, respectively. pp65-specific CTLs killed autologous fibroblasts infected with the laboratory strain CMV AD169. The culture conditions were specific as alloreactive T cells were not expanded. Therefore, this approach could be considered in order to generate efficient virus cytolytic T cells to be used as adoptive immunotherapy in transplanted patients.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
1043-0342
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pubmed:author |
pubmed-author:Caillat-ZucmanSophieS,
pubmed-author:CarneiroJ FJF,
pubmed-author:Cavazzana-CalvoMarinaM,
pubmed-author:FischerAlainA,
pubmed-author:GabrielssonSusanneS,
pubmed-author:Hacein-BeySalimaS,
pubmed-author:HaighTraceyT,
pubmed-author:HamelYaminaY,
pubmed-author:JoossKarinK,
pubmed-author:MartinacheChantalC,
pubmed-author:RickinsonAlan BAB
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
13
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
855-66
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:11975851-Adenoviridae,
pubmed-meshheading:11975851-Cell Separation,
pubmed-meshheading:11975851-Dendritic Cells,
pubmed-meshheading:11975851-Fibroblasts,
pubmed-meshheading:11975851-Flow Cytometry,
pubmed-meshheading:11975851-Gene Transfer Techniques,
pubmed-meshheading:11975851-Green Fluorescent Proteins,
pubmed-meshheading:11975851-Humans,
pubmed-meshheading:11975851-Immunotherapy,
pubmed-meshheading:11975851-Interferon-gamma,
pubmed-meshheading:11975851-Luminescent Proteins,
pubmed-meshheading:11975851-Microscopy, Fluorescence,
pubmed-meshheading:11975851-Phosphoproteins,
pubmed-meshheading:11975851-Plasmids,
pubmed-meshheading:11975851-T-Lymphocytes, Cytotoxic,
pubmed-meshheading:11975851-Time Factors,
pubmed-meshheading:11975851-Transduction, Genetic,
pubmed-meshheading:11975851-Viral Matrix Proteins
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pubmed:year |
2002
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pubmed:articleTitle |
Adenovirally transduced dendritic cells induce bispecific cytotoxic T lymphocyte responses against adenovirus and cytomegalovirus pp65 or against adenovirus and Epstein-Barr virus EBNA3C protein: a novel approach for immunotherapy.
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pubmed:affiliation |
INSERM U429, Hôpital Necker-Enfants Malades, 75743 Paris Cedex 15, France.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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