Linked Life Data

11971906

Source:http://linkedlifedata.com/resource/pubmed/id/11971906

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
29
pubmed:dateCreated
2002-7-15
pubmed:abstractText
MADS-box proteins are transcription factors present in different eukaryotic kingdoms. In contrast to plants, for mammalian and yeast MADS-box proteins ternary complex formation with unrelated transcription factors was reported. We show here the first identification of such ternary interaction in plants. A rice seed-specific NF-YB was identified as partner of OsMADS18 by two-hybrid screening. NF-YB contains a histone fold motif, HFM,(1) and is part of the trimeric CCAAT-binding NF-Y complex. OsMADS18, alone or in combination with a natural partner, interacts with OsNF-YB1 through the MADS and I regions. The mouse NF-YB also associates with OsMADS18 in vivo and in vitro as a NF-YB-NF-YC dimer. Other rice MADS-box proteins do not interact in these assays, indicating specificity for the interaction. OsNF-YB1 is capable of heterodimerizing with NF-YC, but not trimerizing with NF-YA, thus precluding CCAAT binding. Mutation of the variant Asp at position 99 of the HFM alpha2-helix into a conserved serine recovers the capacity to interact with NF-YA, but not with DNA. This is the first indication that members of the NF-YB family work through mechanisms independent of the CCAAT box.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0021-9258
pubmed:author
pubmed:issnType
Print
pubmed:day
19
pubmed:volume
277
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
26429-35
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:11971906-Amino Acid Sequence, pubmed-meshheading:11971906-Animals, pubmed-meshheading:11971906-Arabidopsis, pubmed-meshheading:11971906-CCAAT-Binding Factor, pubmed-meshheading:11971906-DNA-Binding Proteins, pubmed-meshheading:11971906-MADS Domain Proteins, pubmed-meshheading:11971906-Mice, pubmed-meshheading:11971906-Molecular Sequence Data, pubmed-meshheading:11971906-Plant Proteins, pubmed-meshheading:11971906-Protein Binding, pubmed-meshheading:11971906-Protein Conformation, pubmed-meshheading:11971906-Protein Structure, Secondary, pubmed-meshheading:11971906-Rabbits, pubmed-meshheading:11971906-Reverse Transcriptase Polymerase Chain Reaction, pubmed-meshheading:11971906-Sequence Alignment, pubmed-meshheading:11971906-Transcription Factors, pubmed-meshheading:11971906-Two-Hybrid System Techniques
pubmed:year
2002
pubmed:articleTitle
Ternary complex formation between MADS-box transcription factors and the histone fold protein NF-YB.
pubmed:affiliation
Dipartimento di Genetica e Biologia dei Microrganismi, Universitá di Milano, Via Celoria 26, 20133 Milano, Italy.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't