|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
3
|
|
pubmed:dateCreated |
2002-4-23
|
|
pubmed:abstractText |
Beta-endorphin and the synthetic beta-endorphin-like decapeptide Ser-Leu-Thr-Cys-Leu-Val-Lys-Gly-Phe-Tyr (referred to as immunorphin), corresponding to the sequence 364-373 of the CH3 domain of human immunoglobulin G heavy chain, were shown to stimulate concanavalin A-induced proliferation of T lymphocytes from the blood of healthy donors. [Met(5)]Enkephalin and the antagonist of opioid receptors naloxone examined in parallel were inactive. The stimulating effect of beta-endorphin and immunorphin on T lymphocyte proliferation is not inhibited by naloxone. Studies on receptor binding of (125)I-labeled immunorphin to T lymphocytes revealed that it binds with high affinity to naloxone-insensitive receptors (K(d) = 7.0 +/- 0.3 nM). Unlabeled immunorphin completely inhibits (125)I-labeled beta-endorphin specific binding to naloxone-insensitive receptors on T lymphocytes (K(i) = 0.6 +/- 0.1 nM). Thus, beta-endorphin and immunorphin interact with common naloxone-insensitive receptors on T lymphocytes.
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Concanavalin A,
http://linkedlifedata.com/resource/pubmed/chemical/Enkephalin, Methionine,
http://linkedlifedata.com/resource/pubmed/chemical/Immunoglobulin Constant Regions,
http://linkedlifedata.com/resource/pubmed/chemical/Immunoglobulin G,
http://linkedlifedata.com/resource/pubmed/chemical/Immunoglobulin Heavy Chains,
http://linkedlifedata.com/resource/pubmed/chemical/Immunoglobulin gamma-Chains,
http://linkedlifedata.com/resource/pubmed/chemical/Iodine Radioisotopes,
http://linkedlifedata.com/resource/pubmed/chemical/Naloxone,
http://linkedlifedata.com/resource/pubmed/chemical/Narcotic Antagonists,
http://linkedlifedata.com/resource/pubmed/chemical/Oligopeptides,
http://linkedlifedata.com/resource/pubmed/chemical/Peptide Fragments,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Opioid,
http://linkedlifedata.com/resource/pubmed/chemical/beta-Endorphin,
http://linkedlifedata.com/resource/pubmed/chemical/immunorphin
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Mar
|
|
pubmed:issn |
0006-2979
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:volume |
67
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
357-63
|
|
pubmed:dateRevised |
2006-11-15
|
|
pubmed:meshHeading |
pubmed-meshheading:11970735-Binding, Competitive,
pubmed-meshheading:11970735-Cell Division,
pubmed-meshheading:11970735-Cell Transformation, Neoplastic,
pubmed-meshheading:11970735-Concanavalin A,
pubmed-meshheading:11970735-Enkephalin, Methionine,
pubmed-meshheading:11970735-Humans,
pubmed-meshheading:11970735-Immunoglobulin Constant Regions,
pubmed-meshheading:11970735-Immunoglobulin G,
pubmed-meshheading:11970735-Immunoglobulin Heavy Chains,
pubmed-meshheading:11970735-Immunoglobulin gamma-Chains,
pubmed-meshheading:11970735-Iodine Radioisotopes,
pubmed-meshheading:11970735-Lymphocyte Activation,
pubmed-meshheading:11970735-Naloxone,
pubmed-meshheading:11970735-Narcotic Antagonists,
pubmed-meshheading:11970735-Oligopeptides,
pubmed-meshheading:11970735-Peptide Fragments,
pubmed-meshheading:11970735-Receptors, Opioid,
pubmed-meshheading:11970735-T-Lymphocytes,
pubmed-meshheading:11970735-beta-Endorphin
|
|
pubmed:year |
2002
|
|
pubmed:articleTitle |
Effect of synthetic beta-endorphin-like peptide immunorphin on human T lymphocytes.
|
|
pubmed:affiliation |
Branch of the Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, pr. Nauki 6, Pushchino, Moscow Region, 142290 Russia. navolots@fibkh.serpukhov.su
|
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|
