|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
26
|
|
pubmed:dateCreated |
2002-6-24
|
|
pubmed:abstractText |
The Xvent family of homeobox transcription factors is essential for the establishment of the dorsal-ventral body axis during Xenopus embryogenesis. In contrast to Xvent-2B and other members of the Xvent-2 subfamily, Xvent-1B is not a direct response gene of bone morphogenetic protein-4 signaling. Xvent-1B is activated by Xvent-2, but CHX experiments revealed the requirement of additional factors. In this study, we report on the cooperative effect of Xvent-2 and the zinc finger transcription factor GATA-2 on the promoter of the Xvent-1B gene. We show that GATA-2 is a direct target gene of bone morphogenetic protein-4 and that GATA-2 interacts with Xvent-2 to activate transcription of Xvent-1B. Both transcription factors bind to distinct elements within the Xvent-1B promoter, and GATA-2 physically interacts with the C-terminal domain of Xvent-2. Promoter/reporter studies in Xenopus embryos revealed that full activation of Xvent-1B requires both Xvent-2 and GATA-2. Moreover, the two factors are sufficient to direct transcription of Xvent-1B in the presence of CHX at the ventral side of the embryo. The failure of both factors to activate Xvent-1B on the dorsal side suggests the existence of a dorsal inhibitor. This inhibitor is likely a component of the dorsal Wnt signaling pathway because nuclear translocation of beta-catenin before midblastula transition results in a suppression of Xvent-1B transcription.
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Bone Morphogenetic Protein 4,
http://linkedlifedata.com/resource/pubmed/chemical/Bone Morphogenetic Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Cycloheximide,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/GATA2 Transcription Factor,
http://linkedlifedata.com/resource/pubmed/chemical/GATA2 protein, Xenopus,
http://linkedlifedata.com/resource/pubmed/chemical/Homeodomain Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors,
http://linkedlifedata.com/resource/pubmed/chemical/Wnt Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Xenopus Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Xvent-1 protein, Xenopus,
http://linkedlifedata.com/resource/pubmed/chemical/Xvent-2 protein, Xenopus,
http://linkedlifedata.com/resource/pubmed/chemical/Zebrafish Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/bmp4 protein, Xenopus,
http://linkedlifedata.com/resource/pubmed/chemical/bmp4 protein, zebrafish
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Jun
|
|
pubmed:issn |
0021-9258
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:day |
28
|
|
pubmed:volume |
277
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
23872-81
|
|
pubmed:dateRevised |
2008-11-21
|
|
pubmed:meshHeading |
pubmed-meshheading:11964398-Animals,
pubmed-meshheading:11964398-Base Sequence,
pubmed-meshheading:11964398-Bone Morphogenetic Protein 4,
pubmed-meshheading:11964398-Bone Morphogenetic Proteins,
pubmed-meshheading:11964398-Cycloheximide,
pubmed-meshheading:11964398-DNA-Binding Proteins,
pubmed-meshheading:11964398-Female,
pubmed-meshheading:11964398-GATA2 Transcription Factor,
pubmed-meshheading:11964398-Gene Expression Regulation,
pubmed-meshheading:11964398-Genes, Homeobox,
pubmed-meshheading:11964398-Homeodomain Proteins,
pubmed-meshheading:11964398-Molecular Sequence Data,
pubmed-meshheading:11964398-Promoter Regions, Genetic,
pubmed-meshheading:11964398-Proto-Oncogene Proteins,
pubmed-meshheading:11964398-Transcription Factors,
pubmed-meshheading:11964398-Wnt Proteins,
pubmed-meshheading:11964398-Xenopus,
pubmed-meshheading:11964398-Xenopus Proteins,
pubmed-meshheading:11964398-Zebrafish Proteins
|
|
pubmed:year |
2002
|
|
pubmed:articleTitle |
Cooperative interaction of Xvent-2 and GATA-2 in the activation of the ventral homeobox gene Xvent-1B.
|
|
pubmed:affiliation |
Abteilung Biochemie, Universität Ulm, Albert-Einstein Allee 11, Ulm 89081, Germany.
|
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|
