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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
5
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pubmed:dateCreated |
2002-5-6
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pubmed:abstractText |
Several cell surface eukaryotic proteins have a glycosylphosphatidylinositol (GPI) modification at the C-terminal end that serves as an anchor to the plasma membrane and could be responsible for the presence of GPI proteins in rafts, a type of functionally important membrane microdomain enriched in sphingolipids and cholesterol. In order to understand better how GPI proteins partition into rafts, the insertion of the GPI-anchored alkaline phosphatase (AP) was studied in real-time using atomic force microscopy. Supported phospholipid bilayers made of a mixture of sphingomyelin-dioleoylphosphatidylcholine containing cholesterol (Chl+) or not (Chl-) were used to mimic the fluid-ordered lipid phase separation in biological membranes. Spontaneous insertion of AP through its GPI anchor was observed inside both Chl+ and Chl- lipid ordered domains, but AP insertion was markedly increased by the presence of cholesterol.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/11964385-10469152,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11964385-10580089,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11964385-10601014,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11964385-10770957,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11964385-11053133,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11964385-11215618,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11964385-11222302,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11964385-11337891,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11964385-11413487,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11964385-11423436,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11964385-11457463,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11964385-11527149,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11964385-11535814,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11964385-11717303,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11964385-1411505,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11964385-1531449,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11964385-3343238,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11964385-3948290,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11964385-7991596,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11964385-836830,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11964385-9094323,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11964385-9195750,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11964385-9266173,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11964385-9392424,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11964385-9432006
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
1469-221X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
3
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
485-90
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
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pubmed:year |
2002
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pubmed:articleTitle |
Spontaneous insertion and partitioning of alkaline phosphatase into model lipid rafts.
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pubmed:affiliation |
Centre de Biochimie Structurale, CNRS UMR 5048 Université Montpellier I, INSERM UMR554, 29 rue de Navacelles, F-34090 Montpellier cedex, France.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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