Source:http://linkedlifedata.com/resource/pubmed/id/11964083
Switch to
Predicate | Object |
---|---|
rdf:type | |
lifeskim:mentions | |
pubmed:issue |
2
|
pubmed:dateCreated |
2002-4-19
|
pubmed:abstractText |
Patients with pancreatic cancer frequently suffer from thrombosis due to excess thrombin generation. Yet, the effects of thrombin on pancreatic cancer are still poorly understood. The thrombin receptor PAR-1 is responsible for cellular effects of thrombin. PAR-1 plays an important role in the progression of different solid tumours in vitro. In breast cancer the level of PAR-1 expression correlates with invasiveness. Our aim was to correlate PAR-1 mRNA and protein expression level with the grade of differentiation of pancreatic tissue and cancer cell lines. PAR-1 protein was not detectable in the epithelium of healthy pancreas. Analysis of PAR-1 protein expression by immunofluorescence staining of pancreatic cancer cell lines revealed a correlation to the grade of differentiation. Quantitative analysis of PAR-1 protein expression by Western Blot analysis confirmed these observations. Analysis of PAR-1 mRNA expression showed low levels in healthy pancreas compared to pancreatic cancer tissue and the pancreatic cancer cell line MIA PaCa-2. The level of PAR-1 mRNA differed up to 25 fold between the respective pancreatic cancer cell lines. The eminent differences in PAR-1 expression, both protein and mRNA, between healthy pancreatic tissue and pancreatic cancer in vivo and in vitro emphasise the putative role of PAR-1 in pancreatic cancer progression.
|
pubmed:language |
eng
|
pubmed:journal | |
pubmed:citationSubset |
IM
|
pubmed:chemical | |
pubmed:status |
MEDLINE
|
pubmed:issn |
0262-0898
|
pubmed:author |
pubmed-author:BrockmannMichaelM,
pubmed-author:KaufmannRolandR,
pubmed-author:NeugebauerEdmund A MEA,
pubmed-author:ReiseKathrinK,
pubmed-author:RudroffClaudiaC,
pubmed-author:SchäferUteU,
pubmed-author:ScheeleJohannesJ,
pubmed-author:SchneiderAnnetteA,
pubmed-author:SeiboldStefanS,
pubmed-author:ZetinaCecilia CuCC
|
pubmed:issnType |
Print
|
pubmed:volume |
19
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
181-9
|
pubmed:dateRevised |
2006-11-15
|
pubmed:meshHeading |
pubmed-meshheading:11964083-Adenocarcinoma,
pubmed-meshheading:11964083-Blotting, Western,
pubmed-meshheading:11964083-Breast Neoplasms,
pubmed-meshheading:11964083-Cell Differentiation,
pubmed-meshheading:11964083-DNA Primers,
pubmed-meshheading:11964083-Female,
pubmed-meshheading:11964083-Fluorescent Antibody Technique,
pubmed-meshheading:11964083-Humans,
pubmed-meshheading:11964083-Pancreatic Neoplasms,
pubmed-meshheading:11964083-RNA, Messenger,
pubmed-meshheading:11964083-Receptor, PAR-1,
pubmed-meshheading:11964083-Receptors, Thrombin,
pubmed-meshheading:11964083-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:11964083-Tumor Cells, Cultured
|
pubmed:year |
2002
|
pubmed:articleTitle |
Expression of the thrombin receptor PAR-1 correlates with tumour cell differentiation of pancreatic adenocarcinoma in vitro.
|
pubmed:affiliation |
Second Department of Surgery, University of Cologne, Germany. c.rudroff@netcologne.de
|
pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
|