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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
6882
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pubmed:dateCreated |
2002-4-18
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pubmed:databankReference |
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pubmed:abstractText |
SCF complexes are the largest family of E3 ubiquitin-protein ligases and mediate the ubiquitination of diverse regulatory and signalling proteins. Here we present the crystal structure of the Cul1-Rbx1-Skp1-F boxSkp2 SCF complex, which shows that Cul1 is an elongated protein that consists of a long stalk and a globular domain. The globular domain binds the RING finger protein Rbx1 through an intermolecular beta-sheet, forming a two-subunit catalytic core that recruits the ubiquitin-conjugating enzyme. The long stalk, which consists of three repeats of a novel five-helix motif, binds the Skp1-F boxSkp2 protein substrate-recognition complex at its tip. Cul1 serves as a rigid scaffold that organizes the Skp1-F boxSkp2 and Rbx1 subunits, holding them over 100 A apart. The structure suggests that Cul1 may contribute to catalysis through the positioning of the substrate and the ubiquitin-conjugating enzyme, and this model is supported by Cul1 mutations designed to eliminate the rigidity of the scaffold.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Cell Cycle Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Cullin 1,
http://linkedlifedata.com/resource/pubmed/chemical/Cullin Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclin-Dependent Kinase Inhibitor...,
http://linkedlifedata.com/resource/pubmed/chemical/Ligases,
http://linkedlifedata.com/resource/pubmed/chemical/Macromolecular Substances,
http://linkedlifedata.com/resource/pubmed/chemical/NEDD8 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Peptide Synthases,
http://linkedlifedata.com/resource/pubmed/chemical/S-Phase Kinase-Associated Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/SKP Cullin F-Box Protein Ligases,
http://linkedlifedata.com/resource/pubmed/chemical/Tumor Suppressor Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Ubiquitin,
http://linkedlifedata.com/resource/pubmed/chemical/Ubiquitin-Conjugating Enzymes,
http://linkedlifedata.com/resource/pubmed/chemical/Ubiquitin-Protein Ligases,
http://linkedlifedata.com/resource/pubmed/chemical/Ubiquitins
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0028-0836
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pubmed:author |
pubmed-author:ChuClaireC,
pubmed-author:ConawayJoan WJW,
pubmed-author:ConawayRonald CRC,
pubmed-author:ElledgeStephen JSJ,
pubmed-author:HarperJ WadeJW,
pubmed-author:JeffreyPhilip DPD,
pubmed-author:KoeppDeanna MDM,
pubmed-author:MillerJulie JJJ,
pubmed-author:PaganoMicheleM,
pubmed-author:PavletichNikola PNP,
pubmed-author:SchulmanBrenda ABA,
pubmed-author:SongLangzhouL,
pubmed-author:WangPingP,
pubmed-author:ZhengNingN
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pubmed:issnType |
Print
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pubmed:day |
18
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pubmed:volume |
416
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
703-9
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pubmed:dateRevised |
2010-12-17
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pubmed:meshHeading |
pubmed-meshheading:11961546-Amino Acid Motifs,
pubmed-meshheading:11961546-Amino Acid Sequence,
pubmed-meshheading:11961546-Binding Sites,
pubmed-meshheading:11961546-Cell Cycle Proteins,
pubmed-meshheading:11961546-Crystallography, X-Ray,
pubmed-meshheading:11961546-Cullin Proteins,
pubmed-meshheading:11961546-Cyclin-Dependent Kinase Inhibitor p27,
pubmed-meshheading:11961546-Humans,
pubmed-meshheading:11961546-Ligases,
pubmed-meshheading:11961546-Macromolecular Substances,
pubmed-meshheading:11961546-Models, Molecular,
pubmed-meshheading:11961546-Molecular Sequence Data,
pubmed-meshheading:11961546-Peptide Synthases,
pubmed-meshheading:11961546-Protein Binding,
pubmed-meshheading:11961546-Protein Structure, Quaternary,
pubmed-meshheading:11961546-Protein Structure, Tertiary,
pubmed-meshheading:11961546-S-Phase Kinase-Associated Proteins,
pubmed-meshheading:11961546-SKP Cullin F-Box Protein Ligases,
pubmed-meshheading:11961546-Sequence Alignment,
pubmed-meshheading:11961546-Sequence Homology, Amino Acid,
pubmed-meshheading:11961546-Structure-Activity Relationship,
pubmed-meshheading:11961546-Tumor Suppressor Proteins,
pubmed-meshheading:11961546-Ubiquitin,
pubmed-meshheading:11961546-Ubiquitin-Conjugating Enzymes,
pubmed-meshheading:11961546-Ubiquitin-Protein Ligases,
pubmed-meshheading:11961546-Ubiquitins
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pubmed:year |
2002
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pubmed:articleTitle |
Structure of the Cul1-Rbx1-Skp1-F boxSkp2 SCF ubiquitin ligase complex.
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pubmed:affiliation |
Cellular Biochemistry and Biophysics Program, Memorial Sloan-Kettering Cancer Center, New York 10021, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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