Linked Life Data

11960472

Source:http://linkedlifedata.com/resource/pubmed/id/11960472

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
16
pubmed:dateCreated
2002-4-18
pubmed:abstractText
Methods for obtaining combinatorial and array-based data as a function of temperature are needed in the chemical and biological sciences. It is presently quite difficult to employ temperature as a variable using standard wellplate formats simply because it is very inconvenient to keep each well at a distinct temperature. In microfluidics, however, the situation is very different due to the short length scales involved. In this article, it is shown how a simple linear temperature gradient can be generated across dozens of parallel microfluidic channels simultaneously. This result is exploited to rapidly obtain activation energies from catalytic reactions, melting point transitions from lipid membranes, and fluorescence quantum yield curves from semiconductor nanocrystal probes as a function of temperature. The methods developed here could quite easily be extended to protein crystallization, phase diagram measurements, chemical reaction optimization, or multivariable experiments.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0002-7863
pubmed:author
pubmed:issnType
Print
pubmed:day
24
pubmed:volume
124
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
4432-5
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
2002
pubmed:articleTitle
A microfluidic device with a linear temperature gradient for parallel and combinatorial measurements.
pubmed:affiliation
Department of Chemistry, Texas A & M University, College Station, Texas 77843, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, Non-P.H.S., Research Support, Non-U.S. Gov't