Source:http://linkedlifedata.com/resource/pubmed/id/11959688
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
5
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| pubmed:dateCreated |
2002-4-17
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| pubmed:abstractText |
Negative chronotropic and smooth muscle contractile responses to the nonselective muscarinic agonist carbamylcholine were compared in isolated tissues from M(3)-muscarinic receptor knockout and wild-type mice. Carbamylcholine (10(-8)-3.0 x 10(-5) M) induced a concentration-dependent decrease in atrial rate that was similar in atria from M(3)-receptor knockout and wild-type mice, indicating that M(3) receptors were not involved in muscarinic receptor-mediated atrial rate decreases. In contrast, the M(3) receptor was a major muscarinic receptor involved in smooth muscle contraction of stomach fundus, urinary bladder, and trachea, although differences existed in the extent of M(3)-receptor involvement among the tissues. Contraction to carbamylcholine was virtually abolished in urinary bladder from M(3)-receptor knockout mice, suggesting that contraction was predominantly due to M(3)-receptor activation. However, approximately 50-60% maximal contraction to carbamylcholine occurred in stomach fundus and trachea from M(3)-receptor knockout mice, indicating that contraction in these tissues was also due to M(2)-receptor activation. High concentrations of carbamylcholine relaxed the stomach fundus from M(3)-receptor knockout mice by M(1)-receptor activation. Thus M(3)-receptor knockout mice provided unambiguous evidence that M(3) receptors 1) play no role in carbamylcholine-induced atrial rate reduction, 2) are the predominant receptor mediating carbamylcholine-induced urinary bladder contractility, and 3) share contractile responsibility with M(2) receptors in mouse stomach fundus and trachea.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Carbachol,
http://linkedlifedata.com/resource/pubmed/chemical/Cardiotonic Agents,
http://linkedlifedata.com/resource/pubmed/chemical/Cholinergic Agonists,
http://linkedlifedata.com/resource/pubmed/chemical/Receptor, Muscarinic M3,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Muscarinic
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| pubmed:status |
MEDLINE
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| pubmed:month |
May
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| pubmed:issn |
0363-6119
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:volume |
282
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
R1443-9
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| pubmed:dateRevised |
2006-11-15
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| pubmed:meshHeading |
pubmed-meshheading:11959688-Animals,
pubmed-meshheading:11959688-Atrial Function,
pubmed-meshheading:11959688-Body Weight,
pubmed-meshheading:11959688-Carbachol,
pubmed-meshheading:11959688-Cardiotonic Agents,
pubmed-meshheading:11959688-Cholinergic Agonists,
pubmed-meshheading:11959688-Gastric Fundus,
pubmed-meshheading:11959688-Heart Rate,
pubmed-meshheading:11959688-Male,
pubmed-meshheading:11959688-Mice,
pubmed-meshheading:11959688-Mice, Knockout,
pubmed-meshheading:11959688-Muscle, Smooth,
pubmed-meshheading:11959688-Muscle Contraction,
pubmed-meshheading:11959688-Receptor, Muscarinic M3,
pubmed-meshheading:11959688-Receptors, Muscarinic,
pubmed-meshheading:11959688-Reference Values,
pubmed-meshheading:11959688-Trachea,
pubmed-meshheading:11959688-Urinary Bladder
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| pubmed:year |
2002
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| pubmed:articleTitle |
M(3)-receptor knockout mice: muscarinic receptor function in atria, stomach fundus, urinary bladder, and trachea.
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| pubmed:affiliation |
Neuroscience Research, Lilly Research Laboratories, Eli Lilly and Company, Indianapolis, Indiana 46285, USA. stengel_peter_w@lilly.com
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| pubmed:publicationType |
Journal Article
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