Source:http://linkedlifedata.com/resource/pubmed/id/11956200
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
26
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pubmed:dateCreated |
2002-6-24
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pubmed:abstractText |
Mohr-Tranebjaerg syndrome is a progressive, neurodegenerative disorder caused by loss-of-function mutations in the DDP1/TIMM8A gene. DDP1 belongs to a family of evolutionary conserved proteins that are organized in hetero-oligomeric complexes in the mitochondrial intermembrane space. They mediate the import and insertion of hydrophobic membrane proteins into the mitochondrial inner membrane. All of them share a conserved Cys(4) metal binding site proposed to be required for the formation of zinc fingers. So far, the only missense mutation known to cause a full-blown clinical phenotype is a C66W exchange directly affecting this Cys(4) motif. Here, we show that the mutant human protein is efficiently imported into mitochondria and sorted into the intermembrane space. In contrast to wild-type DDP1, it does not complement the function of its yeast homologue Tim8. The C66W mutation impairs binding of Zn(2+) ions via the Cys(4) motif. As a consequence, the mutated DDP1 is incorrectly folded and loses its ability to assemble into a hetero-hexameric 70-kDa complex with its cognate partner protein human Tim13. Thus, an assembly defect of DDP1 is the molecular basis of Mohr-Tranebjaerg syndrome in patients carrying the C66W mutation.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Carrier Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Transport Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Mitochondrial Membrane Transport...,
http://linkedlifedata.com/resource/pubmed/chemical/Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Saccharomyces cerevisiae Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/TIM13 protein, S cerevisiae,
http://linkedlifedata.com/resource/pubmed/chemical/TIMM13 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/TIMM8A protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Zinc
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0021-9258
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:day |
28
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pubmed:volume |
277
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
23287-93
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:11956200-Amino Acid Motifs,
pubmed-meshheading:11956200-Animals,
pubmed-meshheading:11956200-Carrier Proteins,
pubmed-meshheading:11956200-Humans,
pubmed-meshheading:11956200-Membrane Transport Proteins,
pubmed-meshheading:11956200-Mitochondria,
pubmed-meshheading:11956200-Mitochondrial Membrane Transport Proteins,
pubmed-meshheading:11956200-Mutation,
pubmed-meshheading:11956200-Protein Folding,
pubmed-meshheading:11956200-Proteins,
pubmed-meshheading:11956200-Rabbits,
pubmed-meshheading:11956200-Saccharomyces cerevisiae Proteins,
pubmed-meshheading:11956200-Zinc,
pubmed-meshheading:11956200-Zinc Fingers
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pubmed:year |
2002
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pubmed:articleTitle |
The C66W mutation in the deafness dystonia peptide 1 (DDP1) affects the formation of functional DDP1.TIM13 complexes in the mitochondrial intermembrane space.
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pubmed:affiliation |
Institut für Klinische Chemie, Molekulare Diagnostik und Mitochondriale Genetik, Institut für Diabetesforschung und Metabolic Disease Center München-Schwabing, Akademisches Lehrkrankenhaus München-Schwabing, Koelner Platz 1, München 80804, Germany.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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