Source:http://linkedlifedata.com/resource/pubmed/id/11955445
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1
|
| pubmed:dateCreated |
2002-4-16
|
| pubmed:abstractText |
Hox transcription factors have emerged as important regulators of primitive hematopoietic cell proliferation and differentiation. In particular, HOXB4 appears to be a strong positive regulator of hematopoietic stem cell (HSC) self-renewal. Here we demonstrate the potency of HOXB4 to enable high-level ex vivo HSC expansion. Cultures of nontransduced or GFP-transduced murine bone marrow cells experienced large HSC losses over 10-14 days. In sharp contrast, cultures of HOXB4-transduced cells achieved rapid, extensive, and highly polyclonal HSC expansions, resulting in over 1000-fold higher levels relative to controls and a 40-fold net HSC increase. Importantly, these HSCs retained full lympho-myeloid repopulating potential and enhanced in vivo regenerative potential, demonstrating the feasibility of achieving significant ex vivo expansion of HSCs without functional impairment.
|
| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Apr
|
| pubmed:issn |
0092-8674
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| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
5
|
| pubmed:volume |
109
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
39-45
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| pubmed:dateRevised |
2007-11-14
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| pubmed:meshHeading |
pubmed-meshheading:11955445-Age Factors,
pubmed-meshheading:11955445-Animals,
pubmed-meshheading:11955445-Bone Marrow Cells,
pubmed-meshheading:11955445-Bone Marrow Transplantation,
pubmed-meshheading:11955445-Cell Culture Techniques,
pubmed-meshheading:11955445-Cell Division,
pubmed-meshheading:11955445-Clone Cells,
pubmed-meshheading:11955445-Female,
pubmed-meshheading:11955445-Gene Expression Regulation, Developmental,
pubmed-meshheading:11955445-Graft Survival,
pubmed-meshheading:11955445-Hematopoietic Stem Cell Transplantation,
pubmed-meshheading:11955445-Hematopoietic Stem Cells,
pubmed-meshheading:11955445-Homeodomain Proteins,
pubmed-meshheading:11955445-Male,
pubmed-meshheading:11955445-Mice,
pubmed-meshheading:11955445-Mice, Inbred C57BL,
pubmed-meshheading:11955445-Transcription Factors,
pubmed-meshheading:11955445-Transduction, Genetic
|
| pubmed:year |
2002
|
| pubmed:articleTitle |
HOXB4-induced expansion of adult hematopoietic stem cells ex vivo.
|
| pubmed:affiliation |
Terry Fox Laboratory, British Columbia Cancer Agency, 601 West 10th Avenue, V5Z 1L3, Vancouver, British Columbia, Canada.
|
| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
|