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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
5
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pubmed:dateCreated |
2002-4-26
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pubmed:abstractText |
We have investigated the Ca(2+) dependence of vesicular secretion from the soma of dorsal root ganglion (DRG) neurons, which secrete neuropeptides by exocytosis of dense-core vesicles. In patch-clamped somata of rat DRG neurons, we found a depolarization-induced membrane capacitance increase (DeltaC(m)) in the absence of extracellular Ca(2+) and in the presence of a Ca(2+) chelator (BAPTA) in the intracellular solution. Depletion of internal Ca(2+) stores by thapsigargin in the Ca(2+)-free bath also did not block the DeltaC(m), indicating that Ca(2+) release from internal Ca(2+) stores may not have been involved. Furthermore, the Ca(2+)-independent DeltaC(m) was blocked by whole-cell dialysis with tetanus toxin and was accompanied by pulsatile secretion of false transmitters, as detected by amperometric measurements. These results indicate the existence of Ca(2+)-independent but voltage-dependent vesicular secretion (CIVDS) in a mammalian sensory neuron.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/1,2-bis(2-aminophenoxy)ethane-N,N,N'...,
http://linkedlifedata.com/resource/pubmed/chemical/Adenosine Triphosphate,
http://linkedlifedata.com/resource/pubmed/chemical/Anesthetics, Local,
http://linkedlifedata.com/resource/pubmed/chemical/Cadmium,
http://linkedlifedata.com/resource/pubmed/chemical/Calcium,
http://linkedlifedata.com/resource/pubmed/chemical/Calcium Channel Blockers,
http://linkedlifedata.com/resource/pubmed/chemical/Chelating Agents,
http://linkedlifedata.com/resource/pubmed/chemical/Egtazic Acid,
http://linkedlifedata.com/resource/pubmed/chemical/Neuropeptides,
http://linkedlifedata.com/resource/pubmed/chemical/Tetanus Toxin
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
1097-6256
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
5
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
425-30
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:11953753-Action Potentials,
pubmed-meshheading:11953753-Adenosine Triphosphate,
pubmed-meshheading:11953753-Anesthetics, Local,
pubmed-meshheading:11953753-Animals,
pubmed-meshheading:11953753-Cadmium,
pubmed-meshheading:11953753-Calcium,
pubmed-meshheading:11953753-Calcium Channel Blockers,
pubmed-meshheading:11953753-Cell Membrane,
pubmed-meshheading:11953753-Cell Separation,
pubmed-meshheading:11953753-Chelating Agents,
pubmed-meshheading:11953753-Egtazic Acid,
pubmed-meshheading:11953753-Electric Capacitance,
pubmed-meshheading:11953753-Electric Stimulation,
pubmed-meshheading:11953753-Exocytosis,
pubmed-meshheading:11953753-Ganglia, Spinal,
pubmed-meshheading:11953753-Neurons,
pubmed-meshheading:11953753-Neuropeptides,
pubmed-meshheading:11953753-Patch-Clamp Techniques,
pubmed-meshheading:11953753-Rats,
pubmed-meshheading:11953753-Secretory Vesicles,
pubmed-meshheading:11953753-Tetanus Toxin
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pubmed:year |
2002
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pubmed:articleTitle |
Ca(2+)-independent but voltage-dependent secretion in mammalian dorsal root ganglion neurons.
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pubmed:affiliation |
Institute of Neuroscience, Shanghai Institutes of Biological Sciences, Chinese Academy of Sciences, 320 Yue-Yang Road, Shanghai 200031, China.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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