rdf:type |
|
lifeskim:mentions |
|
pubmed:issue |
5
|
pubmed:dateCreated |
2002-4-15
|
pubmed:abstractText |
DC generated from monocytes have been used for vaccines. We have developed a monocyte enrichment procedure by depleting T and B cells with anti-CD2 and anti-CD19 Abs using the automated Isolex 300i magnetic cell selector for clinical-scale DC generation in gas permeable SteriCell culture bags. We have also compared DC function, yield and purity of DC generated from adherent monocytes using culture bags in a closed system, with DC generated in conventional tissue culture flasks.
|
pubmed:language |
eng
|
pubmed:journal |
|
pubmed:citationSubset |
IM
|
pubmed:chemical |
|
pubmed:status |
MEDLINE
|
pubmed:issn |
1465-3249
|
pubmed:author |
|
pubmed:issnType |
Print
|
pubmed:volume |
3
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
365-75
|
pubmed:dateRevised |
2008-4-24
|
pubmed:meshHeading |
pubmed-meshheading:11953016-Antigens, CD19,
pubmed-meshheading:11953016-Antigens, CD2,
pubmed-meshheading:11953016-B-Lymphocytes,
pubmed-meshheading:11953016-Cell Adhesion,
pubmed-meshheading:11953016-Cell Culture Techniques,
pubmed-meshheading:11953016-Cell Division,
pubmed-meshheading:11953016-Cell Separation,
pubmed-meshheading:11953016-Cell Survival,
pubmed-meshheading:11953016-Dendritic Cells,
pubmed-meshheading:11953016-Dextrans,
pubmed-meshheading:11953016-Flow Cytometry,
pubmed-meshheading:11953016-Humans,
pubmed-meshheading:11953016-Immunomagnetic Separation,
pubmed-meshheading:11953016-Immunophenotyping,
pubmed-meshheading:11953016-Monocytes,
pubmed-meshheading:11953016-Pinocytosis,
pubmed-meshheading:11953016-T-Lymphocytes
|
pubmed:year |
2001
|
pubmed:articleTitle |
Comparison of monocyte enrichment by immuno-magnetic depletion or adherence for the clinical-scale generation of DC.
|
pubmed:affiliation |
Nexell Therapeutics, Inc., Cellular Biology, 9 Parker, Irvine, CA 92618, USA.
|
pubmed:publicationType |
Journal Article,
Comparative Study
|