Linked Life Data

11951120

Source:http://linkedlifedata.com/resource/pubmed/id/11951120

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2002-4-12
pubmed:abstractText
The purpose of the present investigation is to study how berberine (Ber) affect the intracellular free calcium concentration ( Ca(2+) (i)) of the smooth muscle cells of guinea pig colon by means of biwavelength spectrophotometry with Fura 2 AM in a cell suspension specimen. In the resting state, Ca(2+) (i) in HEPES Ringer solution (CaCl2 1.5 mmol/L) was 108 9.4 nmol/L (n=7), which was not significantly affected by Ber. On the other hand, Ber inhibited the increased Ca(2+) (i) induced by 60 mmol/L KCl in a dose dependent manner, with a value of IC(50) being 34.09 micromol/L. 30, 100 micromol/L Ber also inhibited 10 micromol/L ACh evoked Ca(2+) (i) elevation in a dose dependent manner either in the presence or absence of extracellular Ca(2+). In addition, Ber inhibited cyclopiazonic acid (CPA) stimulated Ca(2+) (i) elevation dose dependently. This effect was more potent in HEPES Ringer solution (IC(50)=37.79 micromol/L) than in Ca(2+) free medium (IC(50)=49.70 micromol/L). The above results suggest that Ber exerts an inhibitory effect on the extracellular Ca(2+) influx and the Ca(2+) release from intracellular stores.
pubmed:language
chi
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0371-0874
pubmed:author
pubmed:issnType
Print
pubmed:volume
52
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
343-6
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
2000
pubmed:articleTitle
[Effects of berberine on intracellular calcium concentration in smooth muscle cells of guinea pig colon].
pubmed:affiliation
Digestive Disease Research Division, The First Affiliated Hospital of Hubei Medical University, Wuhan 430060, China. Luotang@public.wh.hb.cn
pubmed:publicationType
Journal Article, English Abstract, Research Support, Non-U.S. Gov't