Linked Life Data

11947899

Source:http://linkedlifedata.com/resource/pubmed/id/11947899

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2002-4-11
pubmed:abstractText
The roles of transforming growth factor (TGF)-beta 1 in vascular proliferation, atherosclerosis, and plaque still remain controversial. TGF-beta 1 has been previously reported to inhibit the proliferation and migration of vascular smooth muscle cells and endothelial cells, in vitro. On the other hand, administration or transgenic overexpression of TGF-beta 1 enhances extracellular matrix synthesis and cellular hyperplasia of the intima and media in the normal artery and injured artery in vivo. We evaluated the correlation of arterial proliferation with plasma levels of TGF-beta 1 and TGF-beta receptor type II, respectively, in Otsuka Long-Evans Tokushima Fatty (OLETF) rats, a new strain of spontaneous non-insulin-dependent diabetes mellitus (NIDDM) models. OLETF rats (n=30) were divided into three groups aged 5,15, and 30 weeks. Long-Evans Tokushima Otsuka (LETO) rats (n=30) were used as age-matched non-diabetic controls. Plasma TGF-beta1 and insulin were determined by enzyme-linked immunosorbent assay. Immunoreactive TGF-beta receptor type II antigen was detected by immunohistochemistry on the thoracic artery. Arterial media area was measured microscopically. Oral glucose tolerance test was performed to examine the stage of diabetes mellitus. The thoracic aorta wall section area increased significantly from the age of 15 weeks in OLETF rats, versus LETO rats. In both OLETF and LETO rats, plasma TGF-beta 1 increased significantly from the age of 15 weeks. In OLETF rats, plasma TGF-beta 1 increased significantly over that in LETO rats (P<0.001). Furthermore, TGF-beta receptor type II was detected on aortic wall as strong signals in OLETF rats, but only weakly in LETO rats. OLETF rats showed hyperinsulinemia and insulin resistance from the age of 15 weeks. With oral glucose tolerance test, from the age of 15 weeks, the high glucose level in OLETF rats was prolonged to 2 h after loading, and the insulin levels at both fasting and after loading were significantly higher than those of LETO rats (P<0.001). There are significant linear relations between plasma TGF-beta 1 antigen and aorta wall section area, and plasma TGF-beta 1 antigen and fasting insulin level (P<0.001, respectively). We found that plasma TGF-beta 1 and vascular TGF-beta type II receptors existed to a greater extent in pre- and early stages of diabetes mellitus (DM) in OLETF rats compared with LETO rats. The greater extent of each in OLETF rats was associated with hyperinsulinemia and/or vascular thickening.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0021-9150
pubmed:author
pubmed:issnType
Print
pubmed:volume
162
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
69-76
pubmed:dateRevised
2011-11-17
pubmed:meshHeading
pubmed-meshheading:11947899-Aging, pubmed-meshheading:11947899-Animals, pubmed-meshheading:11947899-Aorta, Thoracic, pubmed-meshheading:11947899-Blood Pressure, pubmed-meshheading:11947899-Body Weight, pubmed-meshheading:11947899-Cholesterol, pubmed-meshheading:11947899-Diabetes Mellitus, pubmed-meshheading:11947899-Disease Models, Animal, pubmed-meshheading:11947899-Endothelium, Vascular, pubmed-meshheading:11947899-Glucose Tolerance Test, pubmed-meshheading:11947899-Immunohistochemistry, pubmed-meshheading:11947899-Insulin, pubmed-meshheading:11947899-Male, pubmed-meshheading:11947899-Muscle, Smooth, Vascular, pubmed-meshheading:11947899-Protein-Serine-Threonine Kinases, pubmed-meshheading:11947899-Rats, pubmed-meshheading:11947899-Rats, Inbred OLETF, pubmed-meshheading:11947899-Rats, Long-Evans, pubmed-meshheading:11947899-Receptors, Transforming Growth Factor beta, pubmed-meshheading:11947899-Time Factors, pubmed-meshheading:11947899-Transforming Growth Factor beta, pubmed-meshheading:11947899-Transforming Growth Factor beta1
pubmed:year
2002
pubmed:articleTitle
Vascular proliferation and transforming growth factor-beta expression in pre- and early stage of diabetes mellitus in Otsuka Long-Evans Tokushima fatty rats.
pubmed:affiliation
Second Department of Internal Medicine, Division of Stroke and Atherosclerosis, Kagawa Medical University, 1750-1 Ikenobe, Miki-cho, Kita-gun, Kagawa 761-0793, Japan. naohisa@kms.ac.jp
pubmed:publicationType
Journal Article, Comparative Study, Evaluation Studies