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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
4
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pubmed:dateCreated |
2002-4-11
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pubmed:abstractText |
Fluid shear stress can activate PI-3 kinase and JNK in vascular endothelial cells. This study was designed to establish the role of Cbl as an upstream molecule in the shear stress activation of PI-3 kinase and JNK. Confluent monolayers of bovine aortic endothelial cells (BAECs) were subjected to a shear stress of 12 dyn/cm(2) over intervals ranging from 0.5 to 30 min. Shear stress increased Cbl phosphorylation to 2.9-fold of control and Cbl association with the regulatory PI-3 kinase subunit p85 to 5.4-fold. The PI-3 kinase activity measured in Cbl-immunoprecipitated complexes increased to 11.7-fold in response to shear, suggesting that the shear stress activation of PI-3 kinase involves its association with Cbl. Furthermore, the shear stress induction of JNK was attenuated by a negative mutant of Cbl. Finally, shear stress caused an activation of PI 3-kinase only in BAECs seeded onto fibronectin, vitronectin, or laminin, but not poly-l-lysine. Our results suggest that Cbl plays a critical role in the shear stress induction of PI 3-kinase and JNK activities, and that this shear-induced activation requires the interaction of endothelial integrins with extracellular matrix proteins.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0006-291X
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pubmed:author |
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pubmed:copyrightInfo |
(c)2002 Elsevier Science (USA).
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pubmed:issnType |
Print
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pubmed:day |
12
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pubmed:volume |
292
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
892-9
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pubmed:dateRevised |
2010-11-18
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pubmed:meshHeading |
pubmed-meshheading:11944898-Animals,
pubmed-meshheading:11944898-Cattle,
pubmed-meshheading:11944898-Cell Membrane,
pubmed-meshheading:11944898-Cells, Cultured,
pubmed-meshheading:11944898-Endothelium, Vascular,
pubmed-meshheading:11944898-Enzyme Activation,
pubmed-meshheading:11944898-Enzyme Induction,
pubmed-meshheading:11944898-Extracellular Matrix,
pubmed-meshheading:11944898-Integrins,
pubmed-meshheading:11944898-JNK Mitogen-Activated Protein Kinases,
pubmed-meshheading:11944898-Mitogen-Activated Protein Kinases,
pubmed-meshheading:11944898-Mutation,
pubmed-meshheading:11944898-Phosphatidylinositol 3-Kinases,
pubmed-meshheading:11944898-Phosphorylation,
pubmed-meshheading:11944898-Precipitin Tests,
pubmed-meshheading:11944898-Protein Transport,
pubmed-meshheading:11944898-Proto-Oncogene Proteins,
pubmed-meshheading:11944898-Proto-Oncogene Proteins c-cbl,
pubmed-meshheading:11944898-Signal Transduction,
pubmed-meshheading:11944898-Stress, Mechanical,
pubmed-meshheading:11944898-Transfection,
pubmed-meshheading:11944898-Tyrosine,
pubmed-meshheading:11944898-Ubiquitin-Protein Ligases
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pubmed:year |
2002
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pubmed:articleTitle |
Role of Cbl in shear-activation of PI 3-kinase and JNK in endothelial cells.
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pubmed:affiliation |
Department of Bioengineering, University of California at San Diego, La Jolla, California 92093-0427, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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