Linked Life Data

11943315

Source:http://linkedlifedata.com/resource/pubmed/id/11943315

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3-4
pubmed:dateCreated
2002-4-10
pubmed:abstractText
An endotoxin-induced mastitis model was used to study permeability changes associated with increased milk matrix metalloproteinase (MMP) activity in early inflammation. One quarter of two cows was inoculated with endotoxin (Escherichia coli 055:B5). Blood, milk, and whey were collected before and repeatedly after inoculation for 48 h. The profile and amounts of gelatinolytic MMPs were determined by zymography; gelatinase A (72 kD MMP-2) and gelatinase B (92 kD MMP-9) were identified by Western immunoblotting. Bovine serum albumin (BSA) and trypsin inhibitor capacity (TIC) were used as markers of capillary permeability with parallel examination of neutrophil penetration from blood to milk. Five clinical E. coli mastitis milk samples and five milk samples from cows with healthy udders were analyzed to detect whether increased levels of gelatinolytic MMP-2 and MMP-9 have a role in naturally occurring mastitis with endotoxin involvement. Milk MMP levels increased 2h after the endotoxin challenge. Both MMP-2 and MMP-9 were involved in this early proteolytic event. These increased MMP levels are associated with increased capillary permeability, evidenced first by the penetration of small molecular weight proteins, such as BSA and TIC. Later, 6-12h post endotoxin inoculation, neutrophilic leucocytes also entered the site, as they require larger tissue damage in basal membrane and interstitial tissue structures than BSA and TIC to extravasate. In naturally occurring disease, increased MMP-2 and MMP-9 levels were detected in milk. Thus, gelatinases, especially MMP-2, are involved in the early degradation of the blood-milk barrier, which precedes the penetration of blood-derived cellular components into milk in endotoxin-induced mastitis. In the future, measuring MMP in milk/whey might be a useful tool for diagnosing early mastitis.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0165-2427
pubmed:author
pubmed:issnType
Print
pubmed:volume
85
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
137-45
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:11943315-Animals, pubmed-meshheading:11943315-Blotting, Western, pubmed-meshheading:11943315-Capillary Permeability, pubmed-meshheading:11943315-Cattle, pubmed-meshheading:11943315-Electrophoresis, Polyacrylamide Gel, pubmed-meshheading:11943315-Endotoxins, pubmed-meshheading:11943315-Escherichia coli, pubmed-meshheading:11943315-Escherichia coli Infections, pubmed-meshheading:11943315-Female, pubmed-meshheading:11943315-Leukocyte Count, pubmed-meshheading:11943315-Mastitis, Bovine, pubmed-meshheading:11943315-Matrix Metalloproteinase 2, pubmed-meshheading:11943315-Matrix Metalloproteinase 9, pubmed-meshheading:11943315-Milk, pubmed-meshheading:11943315-Milk Proteins, pubmed-meshheading:11943315-Plant Proteins, pubmed-meshheading:11943315-Serum Albumin, Bovine, pubmed-meshheading:11943315-Trypsin Inhibitors, pubmed-meshheading:11943315-alpha-Amylases
pubmed:year
2002
pubmed:articleTitle
Increase in milk metalloproteinase activity and vascular permeability in bovine endotoxin-induced and naturally occurring Escherichia coli mastitis.
pubmed:affiliation
Faculty of Veterinary Medicine, Department of Clinical Veterinary Sciences, Helsinki University, P.O. Box 57, Hämeentie 57, Helsinki FIN-00014, Finland.
pubmed:publicationType
Journal Article