Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2002-4-9
pubmed:abstractText
It is a major problem to distinguish between pancreatitis and pancreatic adenocarcinoma when it comes to the perioperative evaluation of pancreatic cryocut sections. In this respect, pathologists are showing a steadily growing interest in the potential application of apoptotic and dedifferentiation factors as diagnostic and prognostic markers. This study investigated the mRNA and protein expression of CD97, CD95 and Fas-L in snap-frozen material obtained from human pancreatic ductal adenocarcinomas (PDC; n = 50), tissues from pancreatitis (PT; n = 40) and normal pancreatic tissues (PN; n = 36). Reverse transcription-polymerase chain reaction (RT-PCR) analysis revealed that CD97, CD95 and Fas-L mRNA were expressed on a similarly high level in all tissues. In contrast, short time immunohistochemical evaluation showed that the CD95 protein was strongly expressed in PT and PN, but not in PDC. Fas-L protein was expressed strongly in PDC, whereas only weak or no expression was noted in PT or PN. CD97 protein expression was detected only in PT and in poorly differentiated PDC. Our data demonstrate that CD97, CD95 and Fas-L can be used as additional markers to distinguish between pancreatitis and pancreatic duct cell carcinoma in cryocut sections.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
1320-5463
pubmed:author
pubmed:issnType
Print
pubmed:volume
52
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
83-8
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:11940212-Antigens, CD, pubmed-meshheading:11940212-Antigens, CD95, pubmed-meshheading:11940212-Carcinoma, Pancreatic Ductal, pubmed-meshheading:11940212-Chronic Disease, pubmed-meshheading:11940212-Diagnosis, Differential, pubmed-meshheading:11940212-Fas Ligand Protein, pubmed-meshheading:11940212-Female, pubmed-meshheading:11940212-Frozen Sections, pubmed-meshheading:11940212-Gene Expression, pubmed-meshheading:11940212-Humans, pubmed-meshheading:11940212-Immunohistochemistry, pubmed-meshheading:11940212-Male, pubmed-meshheading:11940212-Membrane Glycoproteins, pubmed-meshheading:11940212-Membrane Proteins, pubmed-meshheading:11940212-Middle Aged, pubmed-meshheading:11940212-Pancreatectomy, pubmed-meshheading:11940212-Pancreatic Neoplasms, pubmed-meshheading:11940212-Pancreatitis, pubmed-meshheading:11940212-RNA, Messenger
pubmed:year
2002
pubmed:articleTitle
CD97, CD95 and Fas-L clearly discriminate between chronic pancreatitis and pancreatic ductal adenocarcinoma in perioperative evaluation of cryocut sections.
pubmed:affiliation
Department of Pathology, Otto-von-Guericke-University, Magdeburg, Germany. carsten.boltze@medizin.uni-magdeburg.de
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't