Linked Life Data

1193823

Source:http://linkedlifedata.com/resource/pubmed/id/1193823

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3-4
pubmed:dateCreated
1976-2-19
pubmed:abstractText
Replication of dengue type 2 (D2) viruses was studied in four lymphoblastoid cell lines; Raji, HR1, EB3 and RPMI 6410. The HR1 cell line failed to support D2 growth while the other cell lines showed varying susceptibility. Both D2 strain 16681 and strain New Guinea C (NGC) passaged in LLC-MK2 cells replicated readily in Raji cells, while a high mouse-brain-passaged NGC strain did not. A soluble complement-fixing (SCF) antigen from D2-infected Raji cells showed lines of identity with a D2SCF antigen prepared from infected suckling mouse brains. Electron microscopic studies of a D2 Raji carrier culture showed that virions were located in a membrane-vesicle complex in the cytoplasm of the cells. Precursors of viral particles were more frequently observed in the parts of the vesicle which had rough endoplasmic reticular membranes. Mature virus particles were observed often at the boundary of the other part of the vesicle, which consisted of smooth endoplasmic reticulum. Occasionally, a large crystalloid structure consisting of incomplete viral particles was seen in degenerative cells. Dengue carrier cultures in human lymphoblastoid lines may provide a convenient in vitro system for study of aspects of dengue virus-leukocyte interactions.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0300-5526
pubmed:author
pubmed:issnType
Print
pubmed:volume
5
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
137-49
pubmed:dateRevised
2009-10-27
pubmed:meshHeading
pubmed:year
1975
pubmed:articleTitle
Dengue carrier culture and antigen production in human lymphoblastoid lines.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, Non-P.H.S.