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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
8
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pubmed:dateCreated |
2002-4-8
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pubmed:abstractText |
Collagen II (CII)-induced arthritis in DBA/1j mice is mediated by both CII-reactive T cells and anti-CII Ab-producing B cells. To determine the relative role of these processes in the development of arthritis, we specifically eliminated CII-reactive T cells by treating the mice with CII-pulsed syngeneic macrophages that had been transfected with a binary adenovirus system. These macrophages express murine Fas ligand in a doxycycline-inducible manner with autocrine suicide inhibited by concomitant expression of p35. The mice were treated i.v. with four doses of CII-APC-AdFasLp35Tet or a single dose of AdCMVsTACI (5 x 10(9) PFU), or both simultaneously, beginning 2 wk after priming with CII in CFA. Treatment with CII-APC-AdFasLp35Tet alone or in combination with a single dose of AdCMVsTACI prevented the development of CII-induced arthritis and T cell infiltration in the joint. The elimination of T cells was specific in that a normal T cell response was observed on stimulation with OVA after treatment with CII-APC-AdFasLp35Tet. Treatment with AdCMVsTACI alone prevented production of detectable levels of circulating anti-CII autoantibodies and reduced the severity of arthritis but did not prevent its development. These results indicate that the CII-reactive T cells play a crucial role in the development of CII-induced arthritis and that the anti-CII Abs act to enhance the development of CII-induced arthritis.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
AIM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Autoantibodies,
http://linkedlifedata.com/resource/pubmed/chemical/Collagen Type II,
http://linkedlifedata.com/resource/pubmed/chemical/Fas Ligand Protein,
http://linkedlifedata.com/resource/pubmed/chemical/Fasl protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Inhibitor of Apoptosis Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Glycoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Tumor Necrosis Factor,
http://linkedlifedata.com/resource/pubmed/chemical/Tnfrsf13b protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Trans-Activators,
http://linkedlifedata.com/resource/pubmed/chemical/Transmembrane Activator and CAML...,
http://linkedlifedata.com/resource/pubmed/chemical/Viral Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/inhibitor of apoptosis...
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0022-1767
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
168
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
4164-72
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:11937577-Adenoviridae,
pubmed-meshheading:11937577-Administration, Oral,
pubmed-meshheading:11937577-Animals,
pubmed-meshheading:11937577-Antigen-Presenting Cells,
pubmed-meshheading:11937577-Apoptosis,
pubmed-meshheading:11937577-Arthritis, Experimental,
pubmed-meshheading:11937577-Autoantibodies,
pubmed-meshheading:11937577-Autocrine Communication,
pubmed-meshheading:11937577-B-Lymphocytes,
pubmed-meshheading:11937577-Cartilage, Articular,
pubmed-meshheading:11937577-Cell Migration Inhibition,
pubmed-meshheading:11937577-Collagen Type II,
pubmed-meshheading:11937577-Cytomegalovirus,
pubmed-meshheading:11937577-Down-Regulation,
pubmed-meshheading:11937577-Drug Therapy, Combination,
pubmed-meshheading:11937577-Fas Ligand Protein,
pubmed-meshheading:11937577-Female,
pubmed-meshheading:11937577-Genetic Vectors,
pubmed-meshheading:11937577-Inhibitor of Apoptosis Proteins,
pubmed-meshheading:11937577-Lymphocyte Activation,
pubmed-meshheading:11937577-Lymphocyte Depletion,
pubmed-meshheading:11937577-Macrophages, Peritoneal,
pubmed-meshheading:11937577-Membrane Glycoproteins,
pubmed-meshheading:11937577-Membrane Proteins,
pubmed-meshheading:11937577-Mice,
pubmed-meshheading:11937577-Mice, Inbred DBA,
pubmed-meshheading:11937577-Receptors, Tumor Necrosis Factor,
pubmed-meshheading:11937577-Solubility,
pubmed-meshheading:11937577-T-Lymphocyte Subsets,
pubmed-meshheading:11937577-Tetracycline Resistance,
pubmed-meshheading:11937577-Trans-Activators,
pubmed-meshheading:11937577-Transmembrane Activator and CAML Interactor Protein,
pubmed-meshheading:11937577-Viral Proteins
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pubmed:year |
2002
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pubmed:articleTitle |
Depletion of collagen II-reactive T cells and blocking of B cell activation prevents collagen II-induced arthritis in DBA/1j mice.
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pubmed:affiliation |
University of Alabama, Birmingham, AL 35294, USA. Huang-Ge.Zhang@cc.uab.edu
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pubmed:publicationType |
Journal Article
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