Linked Life Data

11937543

Source:http://linkedlifedata.com/resource/pubmed/id/11937543

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
8
pubmed:dateCreated
2002-4-8
pubmed:databankReference
pubmed:abstractText
CD11c is a member of the beta(2) integrin family of adhesion molecules that, together with CD18, forms a heterodimeric receptor on the surface of myeloid, NK, dendritic, and certain leukemic, lymphoma, and activated lymphoid cells. Monocytic differentiation is associated with an induction of both CD11c and CD18 gene expression. The resulting CD11c/CD18 receptor mediates firm adhesion to the vascular endothelium, transendothelial migration, chemotaxis, and phagocytosis. Monocytic differentiation can be mimicked in vitro by treatment of the promonocytic cell line U937 with PMA. Recently, we reported that in U937 cells, expression of the CD11c gene is controlled by an unidentified transcription factor that binds ssDNA. This finding suggested that DNA secondary structure plays an important role in controlling the CD11c gene and prompted us to search for additional ssDNA-binding activities with which this gene interacts. In this study, we report that in U937 cells, expression of the CD11c gene is mediated by the ssDNA-binding protein Puralpha. During PMA-induced differentiation, the ability of Puralpha to activate the CD11c promoter in U937 cells increases, as does that of Sp1. Together, these increases in the functional activity of both Puralpha and Sp1 combine to induce CD11c expression.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0022-1767
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
168
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
3887-93
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:11937543-Antigens, CD18, pubmed-meshheading:11937543-Base Sequence, pubmed-meshheading:11937543-Cell Differentiation, pubmed-meshheading:11937543-Cyclic AMP Response Element-Binding Protein, pubmed-meshheading:11937543-DNA, Single-Stranded, pubmed-meshheading:11937543-DNA-Binding Proteins, pubmed-meshheading:11937543-Gene Expression Regulation, pubmed-meshheading:11937543-Humans, pubmed-meshheading:11937543-Integrin alphaXbeta2, pubmed-meshheading:11937543-Molecular Sequence Data, pubmed-meshheading:11937543-Monocytes, pubmed-meshheading:11937543-Promoter Regions, Genetic, pubmed-meshheading:11937543-Protein Binding, pubmed-meshheading:11937543-Sp1 Transcription Factor, pubmed-meshheading:11937543-Trans-Activators, pubmed-meshheading:11937543-Transcription Factors, pubmed-meshheading:11937543-U937 Cells
pubmed:year
2002
pubmed:articleTitle
During differentiation of the monocytic cell line U937, Pur alpha mediates induction of the CD11c beta 2 integrin gene promoter.
pubmed:affiliation
Renal Unit, Massachusetts General Hospital, Harvard Medical School, Charlestown, MA 02129, USA. shelley@receptor.mgh.harvard.edu
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, U.S. Gov't, Non-P.H.S.