Source:http://linkedlifedata.com/resource/pubmed/id/11933217
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
|
| pubmed:dateCreated |
2002-4-4
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| pubmed:abstractText |
Gene therapy has recently been advanced by the development of HIV-based vectors that are able to transduce some non-dividing cells. The manipulation of most non-dividing cells remains, however, scarcely efficient. One of the biological mechanisms postulated to prevent powerful transduction of quiescent cells by lentiviral vectors is the paucity of deoxynucleotides (dNTPs). In this study, a novel delivery strategy is developed to improve significantly the efficiency of HIV-based vectors in transducing non-dividing cells. This approach is based on increasing the intracellular availability of dNTPs by incubating target cells with the dNTP precursors, deoxynucleosides (dNSs).
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:issn |
1099-498X
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| pubmed:author | |
| pubmed:copyrightInfo |
Copyright 2002 John Wiley & Sons, Ltd.
|
| pubmed:issnType |
Print
|
| pubmed:volume |
4
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
161-9
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| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:11933217-3T3 Cells,
pubmed-meshheading:11933217-Animals,
pubmed-meshheading:11933217-Cell Division,
pubmed-meshheading:11933217-Cell Line,
pubmed-meshheading:11933217-Deoxyribose,
pubmed-meshheading:11933217-Dose-Response Relationship, Drug,
pubmed-meshheading:11933217-Gene Transfer Techniques,
pubmed-meshheading:11933217-Genetic Vectors,
pubmed-meshheading:11933217-HIV,
pubmed-meshheading:11933217-Humans,
pubmed-meshheading:11933217-Lentivirus,
pubmed-meshheading:11933217-Macrophages,
pubmed-meshheading:11933217-Mice,
pubmed-meshheading:11933217-Retroviridae,
pubmed-meshheading:11933217-Tetrazolium Salts,
pubmed-meshheading:11933217-Thiazoles,
pubmed-meshheading:11933217-Time Factors,
pubmed-meshheading:11933217-Transduction, Genetic
|
| pubmed:articleTitle |
High efficiency lentiviral gene delivery in non-dividing cells by deoxynucleoside treatment.
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| pubmed:affiliation |
Research Institute for Genetic and Human Therapy (RIGHT) at IRCCS Policlinico San Matteo, Pavia, Italy.
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|