Source:http://linkedlifedata.com/resource/pubmed/id/11933077
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
4
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| pubmed:dateCreated |
2002-4-4
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| pubmed:abstractText |
Interleukin-1 receptor antagonist (IL-1Ra) is involved in many processes, including epidermal inflammation and hyperplasia after irritation or injury. However, the mechanism by which intracellular IL-1Ra (icIL-1Ra) expression is regulated in mouse keratinocytes has not been reported. We found that the CH72 mouse carcinoma cell line constitutively expresses the icIL-1Ra mRNA. To study the transcriptional factors responsible for the constitutive expression of icIL-1Ra, we functionally characterized 4.5 kb of the 5' flanking region of the human icIL-1Ra gene in these cells. We first demonstrated that icIL-1Ra expression in these cells was regulated at the level of transcription. Deletion analysis of the promoter showed that regulatory elements for constitutive expression were located -158 to -49 bp upstream of the transcription start site for icIL-1Ra. We investigated the cis- and trans-acting factors required for icIL-1Ra expression. An activating protein-1 (AP-1) site was identified as the positive regulatory element necessary for the constitutive expression of the icIL-1Ra promoter in CH72 cells. Moreover, electrophoretic mobility shift assay and cotransfection experiments showed that c-jun and c-fos proteins bound to the AP-1 site and functionally transactivated the icIL-1Ra promoter in mouse carcinoma CH72 cells.
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| pubmed:grant | |
| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/IL1RN protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Il1rn protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin 1 Receptor Antagonist...,
http://linkedlifedata.com/resource/pubmed/chemical/Luciferases,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Sialoglycoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factor AP-1
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| pubmed:status |
MEDLINE
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| pubmed:month |
Apr
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| pubmed:issn |
0899-1987
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| pubmed:author | |
| pubmed:copyrightInfo |
Copyright 2002 Wiley-Liss, Inc.
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| pubmed:issnType |
Print
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| pubmed:volume |
33
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
237-43
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| pubmed:dateRevised |
2007-11-14
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| pubmed:meshHeading |
pubmed-meshheading:11933077-Animals,
pubmed-meshheading:11933077-Base Sequence,
pubmed-meshheading:11933077-Carcinoma, Squamous Cell,
pubmed-meshheading:11933077-Consensus Sequence,
pubmed-meshheading:11933077-Gene Expression Regulation, Neoplastic,
pubmed-meshheading:11933077-Genes, Reporter,
pubmed-meshheading:11933077-Interleukin 1 Receptor Antagonist Protein,
pubmed-meshheading:11933077-Luciferases,
pubmed-meshheading:11933077-Mice,
pubmed-meshheading:11933077-Recombinant Proteins,
pubmed-meshheading:11933077-Sequence Alignment,
pubmed-meshheading:11933077-Sequence Deletion,
pubmed-meshheading:11933077-Sialoglycoproteins,
pubmed-meshheading:11933077-Skin Neoplasms,
pubmed-meshheading:11933077-Transcription, Genetic,
pubmed-meshheading:11933077-Transcription Factor AP-1,
pubmed-meshheading:11933077-Transfection,
pubmed-meshheading:11933077-Tumor Cells, Cultured
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| pubmed:year |
2002
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| pubmed:articleTitle |
Regulation of transcription of the intracellular interleukin-1 receptor antagonist gene by AP-1 in mouse carcinoma cells.
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| pubmed:affiliation |
The University of Texas M. D. Anderson Cancer Center, Science Park-Research Division, Smithville, Texas 78957, USA.
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| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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