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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
4
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pubmed:dateCreated |
2002-4-4
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pubmed:abstractText |
The phosphatidylinositol phosphatase gene PTEN is a dual specific phosphatase acting on phospho amino acids but also on three phosphorylated inositol phospholipids. Present results demonstrate that PTEN is inducible by costimulatory signals in human CD4(+) T cells. PTEN expression was up-regulated on RNA and protein level in freshly isolated human CD4(+) T cells following stimulation with CD28 or CD2. In contrast, PTEN expression was high but remained CD28 and CD2 unresponsive in lymphoma cells. Intracellular staining revealed PTEN expression in CD4(+) T cell populations stimulated with anti-CD28 or anti-CD28 / anti-CD3. Stimulation with anti-CD3 alone did not induce PTEN expression. Inhibition of PTEN expression by antisense oligonucleotides in CD4(+) T cells stimulated with non-mitogenic anti-CD28 resulted in massively increased proliferation, which was sensitive to the phosphatidylinositol 3-kinase (PI3 K) inhibitor wortmannin. Although CD28 and CD2 induce PI3 K signal transduction, wortmannin did not block PTEN up-regulation by CD28 or CD2 indicating that PTEN gene expression is PI3 K independent. These results demonstrate that PTEN negatively controls costimulatory signals by antagonizing PI3 K activity in the absence of TCR engagement.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Androstadienes,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD2,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD28,
http://linkedlifedata.com/resource/pubmed/chemical/Enzyme Inhibitors,
http://linkedlifedata.com/resource/pubmed/chemical/Oligodeoxyribonucleotides, Antisense,
http://linkedlifedata.com/resource/pubmed/chemical/PTEN Phosphohydrolase,
http://linkedlifedata.com/resource/pubmed/chemical/PTEN protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphatidylinositol 3-Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphoric Monoester Hydrolases,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Antigen, T-Cell,
http://linkedlifedata.com/resource/pubmed/chemical/Thionucleotides,
http://linkedlifedata.com/resource/pubmed/chemical/Tumor Suppressor Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/wortmannin
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0014-2980
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
32
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1196-204
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pubmed:dateRevised |
2010-11-18
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pubmed:meshHeading |
pubmed-meshheading:11932928-Adult,
pubmed-meshheading:11932928-Androstadienes,
pubmed-meshheading:11932928-Antigens, CD2,
pubmed-meshheading:11932928-Antigens, CD28,
pubmed-meshheading:11932928-CD4-Positive T-Lymphocytes,
pubmed-meshheading:11932928-Cell Cycle,
pubmed-meshheading:11932928-Cell Division,
pubmed-meshheading:11932928-Cells, Cultured,
pubmed-meshheading:11932928-Enzyme Activation,
pubmed-meshheading:11932928-Enzyme Induction,
pubmed-meshheading:11932928-Enzyme Inhibitors,
pubmed-meshheading:11932928-Humans,
pubmed-meshheading:11932928-Lymphocyte Activation,
pubmed-meshheading:11932928-Lymphoma, T-Cell,
pubmed-meshheading:11932928-Oligodeoxyribonucleotides, Antisense,
pubmed-meshheading:11932928-PTEN Phosphohydrolase,
pubmed-meshheading:11932928-Phosphatidylinositol 3-Kinases,
pubmed-meshheading:11932928-Phosphoric Monoester Hydrolases,
pubmed-meshheading:11932928-RNA, Messenger,
pubmed-meshheading:11932928-Receptors, Antigen, T-Cell,
pubmed-meshheading:11932928-Signal Transduction,
pubmed-meshheading:11932928-Thionucleotides,
pubmed-meshheading:11932928-Tumor Cells, Cultured,
pubmed-meshheading:11932928-Tumor Suppressor Proteins
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pubmed:year |
2002
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pubmed:articleTitle |
The phosphatidylinositol phosphatase PTEN is under control of costimulation and regulates proliferation in human T cells.
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pubmed:affiliation |
Swiss Institute of Allergy and Asthma Research (SIAF), Davos, Switzerland. csweber@siaf.unizh.ch
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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