11924799

Source:http://linkedlifedata.com/resource/pubmed/id/11924799

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Predicate	Object
rdf:type	pubmed:Citation
lifeskim:mentions	umls-concept:C0021311, umls-concept:C0025929, umls-concept:C0220825, umls-concept:C0430022, umls-concept:C1006805
pubmed:issue	5
pubmed:dateCreated	2002-4-1
pubmed:abstractText	Disease-susceptible (C3H) and -resistant (B6) immunocompetent and immunodeficient (C3H-scid and B6-rag1) mice were examined up to 10 weeks after inoculation with Helicobacter bilis (a prototype species of proven virulence). Infection was monitored weekly by use of fecal culture, polymerase chain reaction (PCR) nucleic acid amplification, membrane extract enzyme-linked immunosorbent assay (ELISA), and histologic examination. All mice became infected by three to five weeks after inoculation, on the basis of results of culture and PCR analysis of feces. The PCR analysis was more sensitive than culture at determining infection status, particularly during early infection. None of the mice had evidence of disease by week 10. Immunoglobulin G seroconversion was detectable in C3H mice by week eight and in B6 mice by week nine. Results indicated that culture and PCR analysis are more sensitive than is membrane extract ELISA serologic testing for detecting early infection in individual mice, regardless of genotype or immune status. Results underscore the need for improved seroassays for this important group of murine pathogens.
pubmed:grant	http://linkedlifedata.com/resource/pubmed/grant/RR14034
pubmed:language	eng
pubmed:journal	http://linkedlifedata.com/resource/pubmed/journal/100900466
pubmed:citationSubset	IM
pubmed:chemical	http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Bacterial, http://linkedlifedata.com/resource/pubmed/chemical/DNA, Bacterial, http://linkedlifedata.com/resource/pubmed/chemical/Immunoglobulin G
pubmed:status	MEDLINE
pubmed:month	Oct
pubmed:issn	1532-0820
pubmed:author	pubmed-author:BartholdS WSW, pubmed-author:FengSS, pubmed-author:HodzicEE, pubmed-author:McKisicMM
pubmed:issnType	Print
pubmed:volume	51
pubmed:owner	NLM
pubmed:authorsComplete	Y
pubmed:pagination	406-12
pubmed:dateRevised	2007-11-14
pubmed:meshHeading	pubmed-meshheading:11924799-Adoptive Transfer, pubmed-meshheading:11924799-Animals, pubmed-meshheading:11924799-Antibodies, Bacterial, pubmed-meshheading:11924799-Bacteriological Techniques, pubmed-meshheading:11924799-Base Sequence, pubmed-meshheading:11924799-DNA, Bacterial, pubmed-meshheading:11924799-Enzyme-Linked Immunosorbent Assay, pubmed-meshheading:11924799-Flow Cytometry, pubmed-meshheading:11924799-Helicobacter, pubmed-meshheading:11924799-Helicobacter Infections, pubmed-meshheading:11924799-Immunoglobulin G, pubmed-meshheading:11924799-Mice, pubmed-meshheading:11924799-Mice, Inbred C3H, pubmed-meshheading:11924799-Mice, Inbred C57BL, pubmed-meshheading:11924799-Mice, Knockout, pubmed-meshheading:11924799-Mice, SCID, pubmed-meshheading:11924799-Polymerase Chain Reaction, pubmed-meshheading:11924799-Sensitivity and Specificity, pubmed-meshheading:11924799-Virulence
pubmed:year	2001
pubmed:articleTitle	Evaluation of diagnostic methods for Helicobacter bilis infection in laboratory mice.
pubmed:affiliation	Center for Comparative Medicine, School of Medicine, University of California, Davis 95616, USA.
pubmed:publicationType	Journal Article, Comparative Study, Research Support, U.S. Gov't, P.H.S., Evaluation Studies