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rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
12
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|
pubmed:dateCreated |
1976-2-20
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|
pubmed:abstractText |
Hepatic protein synthesis was investigated using a postmitochondrial supernatant system derived from the livers of rats that were given injections of a single dose of dimethylnitrosamine (DMN), 30 mg/kg. The time course and extent of DMN-induced inhibition in vitro were identical to those reported for the incorporation of amino acids into liver proteins in vivo, maximum inhibition being about 70% at 5 hr. Addition of specific inhibitors of chain initiation (polyinosinic acid and aurin tricarboxylic acid) to the postmitochrondrial supernatant system from DMN-treated rats caused only a slight additional inhibition, indicating that DMN predominantly affects translation by a block of initiation. Treatment with cystamine prior to DMN administration completely abolished the depression of protein synthesis and reduced by more than 90% the methylation by [14C]DMN of purine bases in liver DNA. Pretreatment with pregnenolone-16alpha-carbonitrile stimulated protein synthesis in controls but had no preventive effect in DMN-treated rats and did not reduce the extent of DNA alkylation in vivo.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0008-5472
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pubmed:author |
|
|
pubmed:issnType |
Print
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pubmed:volume |
35
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|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
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pubmed:pagination |
3667-72
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:1192427-Alkylation,
pubmed-meshheading:1192427-Animals,
pubmed-meshheading:1192427-Aurintricarboxylic Acid,
pubmed-meshheading:1192427-Cell-Free System,
pubmed-meshheading:1192427-Cystamine,
pubmed-meshheading:1192427-DNA,
pubmed-meshheading:1192427-Depression, Chemical,
pubmed-meshheading:1192427-Dimethylnitrosamine,
pubmed-meshheading:1192427-Female,
pubmed-meshheading:1192427-Guanine,
pubmed-meshheading:1192427-Liver,
pubmed-meshheading:1192427-Nitrosamines,
pubmed-meshheading:1192427-Poly I,
pubmed-meshheading:1192427-Polyribosomes,
pubmed-meshheading:1192427-Pregnenolone,
pubmed-meshheading:1192427-Protein Biosynthesis,
pubmed-meshheading:1192427-Rats,
pubmed-meshheading:1192427-Time Factors
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|
pubmed:year |
1975
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|
pubmed:articleTitle |
Dimethylnitrosamine-induced inhibition of hepatic protein synthesis in vitro and the effect of pretreatment with cystamine or pregnenolone-16alpha-carbonitrile.
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pubmed:publicationType |
Journal Article,
In Vitro
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