Linked Life Data

11920939

Source:http://linkedlifedata.com/resource/pubmed/id/11920939

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2002-3-28
pubmed:abstractText
One of the mechanisms of the skin blistering effect (vesication) of sulfur mustard (bis-(2-chloroethyl)sulfide, HD) is believed to be via the stimulation of specific protease(s) at the dermal-epidermal junction. Cultured normal human epidermal keratinocytes (NHEK) were used as a model to study and characterize protease stimulated by the mustards 2-chloroethyl ethyl sulfide (CEES), 2-chloro-N-(2-chloroethyl)-N-methylethanamine hydrochloride (nitrogen mustard, HN(2)) and HD. The results obtained using a chromozym (TRY) peptide substrate protease assay revealed the optimum mustard concentrations and time for protease stimulation to be about 200 microM (CEES), 100 microM (HN(2)) and 100 microM (HD) and 16 h. The mustard-stimulated protease was membrane bound and was inhibited by adding a Ca(2+) chelator (either 2 mM EGTA (ethylene glycol-bis(amino ethyl ether) N,N,N',N' tetraacetic acid) or 50 microM BAPTA AM (1,2-bis(z-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid, tetraacetoxy methyl ester) alone or in combination), a serine protease inhibitor diisopropyl fluoro-phosphate (DFP, 1 mM), or a protein synthesis inhibitor cycloheximide (35 microM) in the extracellular medium. These results suggest that mustard toxicity may involve the stimulation of trypsin/chymotrypsin-like serine protease, dependent on Ca(2+) and new protein synthesis. Protein purification by gel exclusion and hydrophobic chromatography produced a 70-80 kDa protease, which had an amino acid sequence homologous with a mammalian-type bacterial serine endopeptidase. Based on this information, research is in progress to identify the protease stimulated by HD in NHEK and to determine whether its inhibitors are useful as prospective antivesicant drugs.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0260-437X
pubmed:author
pubmed:copyrightInfo
Copyright 2002 John Wiley & Sons, Ltd.
pubmed:issnType
Print
pubmed:volume
22
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
139-40
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:11920939-Amino Acid Sequence, pubmed-meshheading:11920939-Blister, pubmed-meshheading:11920939-Cells, Cultured, pubmed-meshheading:11920939-Chelating Agents, pubmed-meshheading:11920939-Chromatography, DEAE-Cellulose, pubmed-meshheading:11920939-Chromatography, Ion Exchange, pubmed-meshheading:11920939-Cycloheximide, pubmed-meshheading:11920939-Dermatologic Agents, pubmed-meshheading:11920939-Drug Therapy, Combination, pubmed-meshheading:11920939-Egtazic Acid, pubmed-meshheading:11920939-Humans, pubmed-meshheading:11920939-Isoflurophate, pubmed-meshheading:11920939-Keratinocytes, pubmed-meshheading:11920939-Mechlorethamine, pubmed-meshheading:11920939-Models, Biological, pubmed-meshheading:11920939-Mustard Gas, pubmed-meshheading:11920939-Poisoning, pubmed-meshheading:11920939-Protease Inhibitors, pubmed-meshheading:11920939-Serine Endopeptidases
pubmed:articleTitle
Sulfur mustard-stimulated protease: a target for antivesicant drugs.
pubmed:affiliation
Biology Department, Division of Experimental Therapeutics, Walter Reed Army Institute of Research, Washington, DC 20307-5100, USA. prabhati.ray@amedd.army.mil
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, Non-P.H.S.