| pubmed-article:11918970 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:11918970 | lifeskim:mentions | umls-concept:C0086418 | lld:lifeskim |
| pubmed-article:11918970 | lifeskim:mentions | umls-concept:C0085262 | lld:lifeskim |
| pubmed-article:11918970 | lifeskim:mentions | umls-concept:C0017786 | lld:lifeskim |
| pubmed-article:11918970 | lifeskim:mentions | umls-concept:C0011209 | lld:lifeskim |
| pubmed-article:11918970 | pubmed:issue | 1 | lld:pubmed |
| pubmed-article:11918970 | pubmed:dateCreated | 2002-3-28 | lld:pubmed |
| pubmed-article:11918970 | pubmed:abstractText | Human glutamate dehydrogenase (GDH) gene was fused with a gene fragment encoding the nine amino acid (RKKRRQRRR) protein transduction domain of human immunodeficiency virus TAT protein in bacterial expression vector to produce genetic in-frame TAT-GDH fusion protein. The TAT-GDH protein can enter PC12 cells efficiently when added exogenously in culture media as determined by Western blot analysis and enzyme activities. Once inside the cells, the transduced denatured TAT-GDH protein showed a full activity of GDH indicating that the TAT-GDH fusion protein was correctly refolded after delivery into cells and the activities of GDH in the TAT-GDH fusion protein was not affected by the addition of the TAT sequence. TAT-GDH fusion protein and TAT itself showed no cytotoxicity in PC12 cells. Although the exact mechanism of transduction across a membrane remains unclear, the transduction activity of TAT-GDH into PC12 cells may suggest new possibilities for direct delivery of GDH into the patients with the GDH-deficient disorders. | lld:pubmed |
| pubmed-article:11918970 | pubmed:language | eng | lld:pubmed |
| pubmed-article:11918970 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11918970 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:11918970 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11918970 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11918970 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11918970 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:11918970 | pubmed:month | Jul | lld:pubmed |
| pubmed-article:11918970 | pubmed:issn | 0197-0186 | lld:pubmed |
| pubmed-article:11918970 | pubmed:author | pubmed-author:ChoiSoo... | lld:pubmed |
| pubmed-article:11918970 | pubmed:author | pubmed-author:ParkJinseuJ | lld:pubmed |
| pubmed-article:11918970 | pubmed:author | pubmed-author:YoonHye-Young... | lld:pubmed |
| pubmed-article:11918970 | pubmed:author | pubmed-author:ChoSung-WooSW | lld:pubmed |
| pubmed-article:11918970 | pubmed:author | pubmed-author:LeeJong EunJE | lld:pubmed |
| pubmed-article:11918970 | pubmed:author | pubmed-author:LeeSung HoSH | lld:pubmed |
| pubmed-article:11918970 | pubmed:author | pubmed-author:YoonChang... | lld:pubmed |
| pubmed-article:11918970 | pubmed:author | pubmed-author:KimTae UeTU | lld:pubmed |
| pubmed-article:11918970 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:11918970 | pubmed:volume | 41 | lld:pubmed |
| pubmed-article:11918970 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:11918970 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:11918970 | pubmed:pagination | 37-42 | lld:pubmed |
| pubmed-article:11918970 | pubmed:dateRevised | 2007-11-15 | lld:pubmed |
| pubmed-article:11918970 | pubmed:meshHeading | pubmed-meshheading:11918970... | lld:pubmed |
| pubmed-article:11918970 | pubmed:meshHeading | pubmed-meshheading:11918970... | lld:pubmed |
| pubmed-article:11918970 | pubmed:meshHeading | pubmed-meshheading:11918970... | lld:pubmed |
| pubmed-article:11918970 | pubmed:meshHeading | pubmed-meshheading:11918970... | lld:pubmed |
| pubmed-article:11918970 | pubmed:meshHeading | pubmed-meshheading:11918970... | lld:pubmed |
| pubmed-article:11918970 | pubmed:meshHeading | pubmed-meshheading:11918970... | lld:pubmed |
| pubmed-article:11918970 | pubmed:meshHeading | pubmed-meshheading:11918970... | lld:pubmed |
| pubmed-article:11918970 | pubmed:meshHeading | pubmed-meshheading:11918970... | lld:pubmed |
| pubmed-article:11918970 | pubmed:meshHeading | pubmed-meshheading:11918970... | lld:pubmed |
| pubmed-article:11918970 | pubmed:year | 2002 | lld:pubmed |
| pubmed-article:11918970 | pubmed:articleTitle | TAT-mediated delivery of human glutamate dehydrogenase into PC12 cells. | lld:pubmed |
| pubmed-article:11918970 | pubmed:affiliation | Department of Biochemistry, University of Ulsan College of Medicine, Seoul, South Korea. | lld:pubmed |
| pubmed-article:11918970 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:11918970 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
