| pubmed-article:11918294 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:11918294 | lifeskim:mentions | umls-concept:C0029431 | lld:lifeskim |
| pubmed-article:11918294 | lifeskim:mentions | umls-concept:C0018956 | lld:lifeskim |
| pubmed-article:11918294 | lifeskim:mentions | umls-concept:C1456820 | lld:lifeskim |
| pubmed-article:11918294 | lifeskim:mentions | umls-concept:C1523116 | lld:lifeskim |
| pubmed-article:11918294 | lifeskim:mentions | umls-concept:C0005533 | lld:lifeskim |
| pubmed-article:11918294 | lifeskim:mentions | umls-concept:C0086597 | lld:lifeskim |
| pubmed-article:11918294 | pubmed:issue | 2 | lld:pubmed |
| pubmed-article:11918294 | pubmed:dateCreated | 2002-3-28 | lld:pubmed |
| pubmed-article:11918294 | pubmed:abstractText | Tumor necrosis factor-alpha (TNF) is a potent osteoclastogenic cytokine that has a fundamental role in the pathogenesis of implant particle-induced osteolysis. The nuclear transcription factor NF-kappaB mediates TNF signaling and this transcription complex is necessary for osteoclastogenesis. Because polymethylmethacrylate (PMMA) particles cause osteolysis, we reasoned the PMMA would induce NF-kappaB activation. In fact, we find that exposure of osteoclast precursors, in the form of colony stimulating factor-1 (CSF-1) dependent murine bone marrow macrophages, to PMMA particles prompts nuclear translocation and activation of NF-kappaB. Supershift assays confirm the presence of the p50 and p65 NF-kappaB subunits in the activated transcription factor. Particle-induced NF-kappaB activation is equal in both wild type and LPS- hyporesponsive cells indicating that the phenomenon does not represent endotoxin contamination. A soluble, competitive inhibitor of TNF (huTNFr:Fc) dampens particle-directed NF-kappaB activation and this response is also abrogated in TNF-/- osteoclast precursors. Thus, PMMA particle activation of NF-kappaB is a secondary event resulting from enhanced TNF expression and is independent of LPS contamination. | lld:pubmed |
| pubmed-article:11918294 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11918294 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11918294 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11918294 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11918294 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11918294 | pubmed:language | eng | lld:pubmed |
| pubmed-article:11918294 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11918294 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:11918294 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11918294 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11918294 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11918294 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11918294 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11918294 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:11918294 | pubmed:month | Mar | lld:pubmed |
| pubmed-article:11918294 | pubmed:issn | 0736-0266 | lld:pubmed |
| pubmed-article:11918294 | pubmed:author | pubmed-author:ChenShaopingS | lld:pubmed |
| pubmed-article:11918294 | pubmed:author | pubmed-author:ClohisyJohn... | lld:pubmed |
| pubmed-article:11918294 | pubmed:author | pubmed-author:TeitelbaumSte... | lld:pubmed |
| pubmed-article:11918294 | pubmed:author | pubmed-author:ErdmannJeanne... | lld:pubmed |
| pubmed-article:11918294 | pubmed:author | pubmed-author:Abu-AmerYouse... | lld:pubmed |
| pubmed-article:11918294 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:11918294 | pubmed:volume | 20 | lld:pubmed |
| pubmed-article:11918294 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:11918294 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:11918294 | pubmed:pagination | 174-81 | lld:pubmed |
| pubmed-article:11918294 | pubmed:dateRevised | 2007-11-14 | lld:pubmed |
| pubmed-article:11918294 | pubmed:meshHeading | pubmed-meshheading:11918294... | lld:pubmed |
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| pubmed-article:11918294 | pubmed:meshHeading | pubmed-meshheading:11918294... | lld:pubmed |
| pubmed-article:11918294 | pubmed:meshHeading | pubmed-meshheading:11918294... | lld:pubmed |
| pubmed-article:11918294 | pubmed:meshHeading | pubmed-meshheading:11918294... | lld:pubmed |
| pubmed-article:11918294 | pubmed:meshHeading | pubmed-meshheading:11918294... | lld:pubmed |
| pubmed-article:11918294 | pubmed:meshHeading | pubmed-meshheading:11918294... | lld:pubmed |
| pubmed-article:11918294 | pubmed:meshHeading | pubmed-meshheading:11918294... | lld:pubmed |
| pubmed-article:11918294 | pubmed:meshHeading | pubmed-meshheading:11918294... | lld:pubmed |
| pubmed-article:11918294 | pubmed:year | 2002 | lld:pubmed |
| pubmed-article:11918294 | pubmed:articleTitle | Tumor necrosis factor-alpha mediates polymethylmethacrylate particle-induced NF-kappaB activation in osteoclast precursor cells. | lld:pubmed |
| pubmed-article:11918294 | pubmed:affiliation | Department of Orthopaedic Surgery, Barnes-Jewish Hospital at Washington University School of Medicine, St. Louis, MO 63110, USA. jclohisy@msnotes.wustl.edu | lld:pubmed |
| pubmed-article:11918294 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:11918294 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:11918294 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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