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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
7
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pubmed:dateCreated |
2002-3-27
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pubmed:abstractText |
Homing endonucleases are enzymes that catalyze the highly sequence-specific cleavage of DNA. We have developed an in vivo selection in Escherichia coli that links cell survival with homing endonuclease-mediated DNA cleavage activity and sequence specificity. Using this selection, wild-type and mutant variants of three homing endonucleases were characterized without requiring protein purification and in vitro analysis. This selection system may facilitate the study of sequence-specific DNA cleaving enzymes, and selections based on this work may enable the evolution of homing endonucleases with novel activities or specificities.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/11917035-10220370,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11917035-10487208,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11917035-10601013,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11917035-10666469,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11917035-10871351,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11917035-11016843,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11917035-11279183,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11917035-11557808,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11917035-1514688,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11917035-2696173,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11917035-2827747,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11917035-2842757,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11917035-3050134,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11917035-7608087,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11917035-8238896,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11917035-8335007,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11917035-8532672,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11917035-8643620,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11917035-8932361,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11917035-9006046,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11917035-9170322,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11917035-9254693,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11917035-9331409,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11917035-9409828,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11917035-9468518
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
1362-4962
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:day |
1
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pubmed:volume |
30
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
e29
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
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pubmed:year |
2002
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pubmed:articleTitle |
An in vivo selection system for homing endonuclease activity.
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pubmed:affiliation |
Department of Chemistry and Chemical Biology, Harvard University, 12 Oxford Street, Cambridge, MA 02138, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't
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