Source:http://linkedlifedata.com/resource/pubmed/id/11916269
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
1
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pubmed:dateCreated |
2002-3-27
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pubmed:abstractText |
Quantification of the new immunosuppressant sirolimus (syn. rapamycin; Rapamune) in whole blood by chromatography is essential for its clinical use since no immunoassay is available although monitoring is mandatory. Here we report on a rapid and convenient liquid chromatography (LC-tandem mass spectrometry method and describe our practical experience with its routine use. Whole blood samples were hemolyzed and deproteinized using an equal volume (150 microl) of a mixture of methanol/zinc sulfate solution containing the internal standard desmethoxy-rapamycin. After centrifugation, the clear supernatants were submitted to an on-line solid-phase extraction procedure using the polymeric Waters Oasis HLB material, with elution of the extracts onto the analytical column in the back-flush mode by column switching. For analytical chromatography a RP-C18 column was used with 90/10 methanol/2 mM ammonium acetate as the mobile phase. A 1:10 split was used for the transfer to the mass spectrometer, a Micromass Quattro LC-tandem mass spectrometry system equipped with a Z-spray source and used in the positive electrospray ionization mode. The following transitions were recorded: sirolimus, 931>864 m/z, and desmethoxy-rapamycin (I.S.), 901>834 m/z. The analytical running time was 5 min, including on-line extraction. The method has a linear calibration curve (r>0.99; range 1.6-50 microg/l) and is rugged and precise with monthly CVs <7% at a sirolimus concentration of 13.1 microg/l in routine use; the instrumentation proved to be reliable and required minimal maintenance. Clin Chem
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
1434-6621
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
40
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
40-5
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:11916269-Chromatography, Liquid,
pubmed-meshheading:11916269-Humans,
pubmed-meshheading:11916269-Immunosuppressive Agents,
pubmed-meshheading:11916269-Mass Spectrometry,
pubmed-meshheading:11916269-Reproducibility of Results,
pubmed-meshheading:11916269-Sensitivity and Specificity,
pubmed-meshheading:11916269-Sirolimus
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pubmed:year |
2002
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pubmed:articleTitle |
Quantification of sirolimus by liquid chromatography-tandem mass spectrometry using on-line solid-phase extraction.
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pubmed:affiliation |
Institute of Clinical Chemistry, Ludwig-Maximilians-Universität München, Klinikum Grosshadern, Munich, Germany. mvogeser@klch.med.uni-muenchen.de
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pubmed:publicationType |
Journal Article
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