Source:http://linkedlifedata.com/resource/pubmed/id/11916259
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
4
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pubmed:dateCreated |
2002-3-27
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pubmed:abstractText |
In human and rat, tissue-specific proteolytic processing of identical proglucagon precursors yield tissue-specific proglucagon-derived peptides. In contrast, in many non-mammalian vertebrates alternative mRNA splicing yields different proglucagon precursors in different tissues. Thus alternative mRNA splicing, in part, limits the choices of proglucagon-derived peptides that can be produced by proteolytic processing. Stomach proglucagon mRNAs from the rainbow trout and Xenopus laevis were found not to encode the proglucagon-derived peptide glucagon-like peptide 2 (GLP-2). To determine if the absence of GLP-2 was a general feature of stomach proglucagons we isolated and characterized proglucagon cDNAs from the stomach and the pancreas of the dog, a mammal that expresses the proglucagon gene in the stomach. A major proglucagon transcript of about 1100 bases and a minor transcript of about 800 bases were identified in both stomach and pancreas. The coding sequences of both the stomach and pancreatic proglucagon transcripts were identical. Therefore, tissue-specific proteolytic processing, and not alternative mRNA splicing, must regulate the production of tissue-specific proglucagon-derived peptides from the stomach of the dog.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
1042-5179
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
12
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
253-60
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:11916259-Amino Acid Sequence,
pubmed-meshheading:11916259-Animals,
pubmed-meshheading:11916259-Base Sequence,
pubmed-meshheading:11916259-Cloning, Molecular,
pubmed-meshheading:11916259-DNA, Complementary,
pubmed-meshheading:11916259-Dogs,
pubmed-meshheading:11916259-Glucagon,
pubmed-meshheading:11916259-Molecular Sequence Data,
pubmed-meshheading:11916259-Pancreas,
pubmed-meshheading:11916259-Proglucagon,
pubmed-meshheading:11916259-Protein Precursors,
pubmed-meshheading:11916259-Sequence Alignment,
pubmed-meshheading:11916259-Sequence Analysis, DNA,
pubmed-meshheading:11916259-Sequence Analysis, RNA,
pubmed-meshheading:11916259-Stomach
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pubmed:year |
2001
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pubmed:articleTitle |
cDNA cloning of proglucagon from the stomach and pancreas of the dog.
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pubmed:affiliation |
Department of Laboratory Medicine and Pathobiology, Faculty of Medicine, Banting and Best Diabetes Centre, University of Toronto, Ont., Canada. david.irwin@utoronto.ca
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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