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rdf:type |
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lifeskim:mentions |
umls-concept:C0000768,
umls-concept:C0085862,
umls-concept:C0105770,
umls-concept:C0125440,
umls-concept:C0181586,
umls-concept:C0205227,
umls-concept:C0205228,
umls-concept:C0332120,
umls-concept:C0521447,
umls-concept:C0699788,
umls-concept:C0851827,
umls-concept:C0887839,
umls-concept:C1299583,
umls-concept:C1549571,
umls-concept:C1608386,
umls-concept:C1701901
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pubmed:issue |
11
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pubmed:dateCreated |
2002-3-26
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pubmed:abstractText |
The once accepted idea that LEF-1 transports beta-catenin into nuclei has recently been challenged by experiments using exogenous beta-catenin. Here, we investigated the effects of beta-catenin and LEF-1 on nuclear import of beta-catenin using different combinations of exogenous and endogenous molecules over longer lengths of time than previously studied. Nuclear beta-catenin is not detectable in corneal fibroblasts and epithelia or NIH 3T3 and MDCK cells. In LEF-1 transfections, we show that the B-box of LEF-1 is required to move cytoplasmic endogenous beta-catenin into the nuclei of such cells, proving that LEF-1 does transport endogenous beta-catenin into nuclei. Moreover, transfection of uveal melanoma cells with B-box deficient LEF-1 inhibits nuclear import of beta-catenin by endogenous LEF-1. However, the movement of overexpressed exogenous beta-catenin into nuclei is unaffected by the presence or absence of LEF-1 and forms abnormal nuclear aggregates that are a prelude to subsequent apoptosis. We conclude that nuclear transport of exogenous beta-catenin independently of LEF-1 has questionable physiological significance.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:issn |
1065-6995
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pubmed:author |
|
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pubmed:issnType |
Print
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pubmed:volume |
25
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1149-61
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:11913959-Active Transport, Cell Nucleus,
pubmed-meshheading:11913959-Cell Nucleus,
pubmed-meshheading:11913959-Cytoskeletal Proteins,
pubmed-meshheading:11913959-DNA-Binding Proteins,
pubmed-meshheading:11913959-Epithelial Cells,
pubmed-meshheading:11913959-Fibroblasts,
pubmed-meshheading:11913959-Fluorescent Antibody Technique,
pubmed-meshheading:11913959-Humans,
pubmed-meshheading:11913959-Lymphoid Enhancer-Binding Factor 1,
pubmed-meshheading:11913959-Trans-Activators,
pubmed-meshheading:11913959-Transcription Factors,
pubmed-meshheading:11913959-Transfection,
pubmed-meshheading:11913959-Tumor Cells, Cultured,
pubmed-meshheading:11913959-beta Catenin
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pubmed:year |
2001
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pubmed:articleTitle |
New evidence that nuclear import of endogenous beta-catenin is LEF-1 dependent, while LEF-1 independent import of exogenous beta-catenin leads to nuclear abnormalities.
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pubmed:affiliation |
Department of Cell Biology, Harvard Medical School, Boston, Massachusetts 02115, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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