Linked Life Data

11912555

Source:http://linkedlifedata.com/resource/pubmed/id/11912555

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2002-3-25
pubmed:abstractText
In obese humans, insulin resistance is accompanied by elevated levels of plasma cell membrane glycoprotein (PC-1) and decreased insulin receptor (IR) tyrosine kinase activity in skeletal muscle. PC-1 overexpression inhibits IR tyrosine kinase and possibly other downstream signaling events. The rhesus monkey in captivity is susceptible to obesity with concomitant insulin resistance. In the present study we analyzed obese (n = 10, 29.4% +/- 1.2% body fat) and non-obese (n = 12, 19.4% +/- 1.9% body fat) rhesus monkeys. Glucose clearance during an euglycemic hyperinsulinemic (400 mU/m(2) body surface area/min) clamp was lower for the obese group (non-obese, 9.7 +/- 0.9; obese, 3.2 +/- 0.7 mg/kg fat-free mass [FFM]/min; P <.01). We performed vastus lateralis muscle biopsies prior to and during the clamp. We measured PC-1 levels in these muscle samples to determine whether PC-1 content is elevated in this primate model of insulin resistance. PC-1 levels were determined by assay of phosphodiesterase activity and specific PC-1 enzyme-linked immunosorbent assay (ELISA). In the obese group, both PC-1 content and activity were 2-fold higher than in the non-obese group (P <.05). In order to investigate the ability of insulin to stimulate IR signaling in vivo in these 2 groups of monkeys, we then measured tyrosine autophosphorylation of the IR by specific ELISA. The increase in IR autophosphorylation in the non-obese group was twice that of the obese group (fold increase over basal: non-obese, 3.7 +/- 0.3; obese, 1.9 +/- 0.6; P <.05). We conclude that insulin resistance secondary to obesity in rhesus monkeys is associated with increased levels of PC-1 and decreased IR signaling capacity in skeletal muscle.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0026-0495
pubmed:author
pubmed:copyrightInfo
Copyright 2002, Elsevier Science (USA). All rights reserved.
pubmed:issnType
Print
pubmed:volume
51
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
465-70
pubmed:dateRevised
2011-11-17
pubmed:meshHeading
pubmed-meshheading:11912555-Adipose Tissue, pubmed-meshheading:11912555-Aging, pubmed-meshheading:11912555-Animals, pubmed-meshheading:11912555-Blood Glucose, pubmed-meshheading:11912555-Body Weight, pubmed-meshheading:11912555-Fasting, pubmed-meshheading:11912555-Glucose Clamp Technique, pubmed-meshheading:11912555-Insulin, pubmed-meshheading:11912555-Insulin Resistance, pubmed-meshheading:11912555-Macaca mulatta, pubmed-meshheading:11912555-Membrane Glycoproteins, pubmed-meshheading:11912555-Muscle, Skeletal, pubmed-meshheading:11912555-Obesity, pubmed-meshheading:11912555-Phosphoric Diester Hydrolases, pubmed-meshheading:11912555-Phosphorylation, pubmed-meshheading:11912555-Protein Tyrosine Phosphatase, Non-Receptor Type 1, pubmed-meshheading:11912555-Protein Tyrosine Phosphatases, pubmed-meshheading:11912555-Pyrophosphatases, pubmed-meshheading:11912555-Receptor, Insulin, pubmed-meshheading:11912555-Reference Values
pubmed:year
2002
pubmed:articleTitle
Elevated plasma cell membrane glycoprotein levels and diminished insulin receptor autophosphorylation in obese, insulin-resistant rhesus monkeys.
pubmed:affiliation
Diabetes and Obesity Research Center, School of Medicine, Department of Physiology, University of Maryland at Baltimore, Baltimore, MD, USA.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't