Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
22
pubmed:dateCreated
2002-5-28
pubmed:abstractText
Using cDNA microarrays, we recently identified a large number of transcripts that are regulated differentially by the c-Myc oncoprotein in myeloid cells. Here, we characterize one of these, termed MT-MC1 (Myc Target in Myeloid Cells-1). MT-MC1 is a widely expressed nuclear protein whose overexpression, unlike that of c-Myc targets reported previously, recapitulates multiple c-Myc phenotypes. These include promotion of apoptosis, alteration of morphology, enhancement of anchorage-independent growth, tumorigenic conversion, promotion of genomic instability, and inhibition of hematopoietic differentiation. The MT-MC1 promoter is a direct c-Myc target; it contains two consensus E-box elements, both of which bind c-Myc.Max heterodimers. Mutation of either site abrogates DNA binding by c-Myc.Max and renders the promoter c-Myc unresponsive. Finally, MT-MC1 regulates the expression of several other c-Myc target genes. MT-MC1 represents a proximal and direct c-Myc target that recapitulates many of the properties typically associated with Myc oncoprotein overexpression.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0021-9258
pubmed:author
pubmed:issnType
Print
pubmed:day
31
pubmed:volume
277
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
19998-20010
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:11909865-Amino Acid Sequence, pubmed-meshheading:11909865-Animals, pubmed-meshheading:11909865-Apoptosis, pubmed-meshheading:11909865-Base Sequence, pubmed-meshheading:11909865-Blotting, Northern, pubmed-meshheading:11909865-Blotting, Western, pubmed-meshheading:11909865-COS Cells, pubmed-meshheading:11909865-Carrier Proteins, pubmed-meshheading:11909865-Cell Differentiation, pubmed-meshheading:11909865-Cell Division, pubmed-meshheading:11909865-Cell Nucleus, pubmed-meshheading:11909865-DNA, Complementary, pubmed-meshheading:11909865-Fibroblasts, pubmed-meshheading:11909865-Flow Cytometry, pubmed-meshheading:11909865-Green Fluorescent Proteins, pubmed-meshheading:11909865-Luminescent Proteins, pubmed-meshheading:11909865-Mice, pubmed-meshheading:11909865-Mice, Nude, pubmed-meshheading:11909865-Microscopy, Fluorescence, pubmed-meshheading:11909865-Models, Genetic, pubmed-meshheading:11909865-Molecular Sequence Data, pubmed-meshheading:11909865-Oligonucleotide Array Sequence Analysis, pubmed-meshheading:11909865-Phenotype, pubmed-meshheading:11909865-Plasmids, pubmed-meshheading:11909865-Promoter Regions, Genetic, pubmed-meshheading:11909865-Protein Structure, Tertiary, pubmed-meshheading:11909865-Proto-Oncogene Proteins c-myc, pubmed-meshheading:11909865-Rats, pubmed-meshheading:11909865-Recombinant Fusion Proteins, pubmed-meshheading:11909865-Time Factors, pubmed-meshheading:11909865-Transfection
pubmed:year
2002
pubmed:articleTitle
Myc target in myeloid cells-1, a novel c-Myc target, recapitulates multiple c-Myc phenotypes.
pubmed:affiliation
Section of Hematology/Oncology, Children's Hospital of Pittsburgh, the Department of Molecular Genetics and Biochemistry, the University of Pittsburgh, and the University of Pittsburgh Cancer Institute, Pittsburgh, Pennsylvania 15213, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.