Linked Life Data

11909863

Source:http://linkedlifedata.com/resource/pubmed/id/11909863

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
22
pubmed:dateCreated
2002-5-28
pubmed:abstractText
Heparan sulfate proteoglycans are thought to mediate the action of growth factors. The heparan sulfate-containing proteoglycans in extracts of the bovine fetal rib growth plate were detected using the monoclonal antibody 3G10, which recognizes a neoepitope generated by heparitinase digestion (David, G., Bai, X. M., Van der Schueren, B., Cassiman, J. J., and Van den Berghe, H. (1992) J. Cell Biol. 119, 961-975). The heparan sulfate proteoglycans that react with this antibody were identified using antisera to known proteoglycans; purified using CsCl density gradient centrifugation, molecular sieve, and ion exchange chromatography; and then characterized. The major heparan sulfate proteoglycans in the growth plate had core proteins of 200 kDa and larger and were identified as perlecan and aggrecan. These two heparan sulfate proteoglycans could be effectively separated from each other by CsCl density gradient centrifugation alone. Perlecan contained 25% heparan sulfate and 75% chondroitin sulfate. The heparan sulfate chains on growth plate perlecan were considerably smaller than the chondroitin sulfate chains, and the heparan sulfate disaccharide content was different than that found for heparan sulfate from either kidney, tumor tissue, or growth plate aggrecan. Aggrecan contained only 0.1% heparan sulfate, which was localized to the CS-1 domain of aggrecan. These results indicate that perlecan and aggrecan would be the principal candidate proteoglycans involved in the action of heparan sulfate-binding proteins in the developing growth plate.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0021-9258
pubmed:author
pubmed:issnType
Print
pubmed:day
31
pubmed:volume
277
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
19461-9
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:11909863-Amino Acid Sequence, pubmed-meshheading:11909863-Animals, pubmed-meshheading:11909863-Blotting, Western, pubmed-meshheading:11909863-Cattle, pubmed-meshheading:11909863-Cesium, pubmed-meshheading:11909863-Chlorides, pubmed-meshheading:11909863-Chondroitin Sulfates, pubmed-meshheading:11909863-Chromatography, Ion Exchange, pubmed-meshheading:11909863-Epitopes, pubmed-meshheading:11909863-Heparan Sulfate Proteoglycans, pubmed-meshheading:11909863-Kidney, pubmed-meshheading:11909863-Molecular Sequence Data, pubmed-meshheading:11909863-Peptides, pubmed-meshheading:11909863-Protein Binding, pubmed-meshheading:11909863-Protein Structure, Tertiary, pubmed-meshheading:11909863-Proteoglycans, pubmed-meshheading:11909863-Ribs, pubmed-meshheading:11909863-Sequence Homology, Amino Acid, pubmed-meshheading:11909863-Time Factors, pubmed-meshheading:11909863-Trypsin
pubmed:year
2002
pubmed:articleTitle
Isolation and identification of the major heparan sulfate proteoglycans in the developing bovine rib growth plate.
pubmed:affiliation
Center for Research in Skeletal Development and Pediatric Orthopedics, Shriners Hospital for Children, Tampa, Florida 33612, USA.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't