Linked Life Data

11907817

Source:http://linkedlifedata.com/resource/pubmed/id/11907817

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2002-3-21
pubmed:abstractText
The spatiotemporal properties of the Ca2+ release process in skeletal muscle fibres were determined using a confocal spot detection system. The low-affinity, fluorescent Ca2+ indicator Oregon Green 488 BAPTA-5N (OGB-5N) was used to record localized, action potential-induced fluorescence signals from consecutive locations separated by 200 nm within a single sarcomere. Three-dimensional reconstructions of the Ca2+ transients illustrated the existence of fluorescence domains around Ca2+ release sites, which are centred at the T-tubules. By constructing isochronal plots, it was estimated that the earliest detectable full width at half-maximum (FWHM) of the Ca2+ domains was 0.77+/-0.08 microm and increased rapidly with time to 1.4+/-0.04 microm at peak (17-18 degrees C). A delay of 0.64+/-0.1 ms was observed between the onset of the fluorescence transients at the Z- and M-lines. Deconvolution of fluorescence transients gave estimates of approximately 9 and 2 microM for the peak [Ca2+] changes at the Z and M-lines, respectively. Our results are compatible with the possibility that action potential stimulation elicits Ca2+ release from a region of the sarcoplasmic reticulum (SR) broader than the T-SR junction.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0031-6768
pubmed:author
pubmed:issnType
Print
pubmed:volume
443
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
508-19
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed:year
2002
pubmed:articleTitle
Characterization of the calcium release domains during excitation-contraction coupling in skeletal muscle fibres.
pubmed:affiliation
Department of Physiology, UCLA School of Medicine, 10833 Le Conte Avenue 53-263 CHS, Los Angeles, CA 90095-1751, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't