Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
8
pubmed:dateCreated
2002-3-21
pubmed:abstractText
The movement protein (MP) of Tobacco mosaic virus (TMV) facilitates the cell-to-cell transport of the viral RNA genome through plasmodesmata (Pd). A previous report described the functional reversion of a dysfunctional mutation in MP (Pro81Ser) by two additional amino acid substitution mutations (Thr104Ile and Arg167Lys). To further explore the mechanism underlying this intramolecular complementation event, the mutations were introduced into a virus derivative expressing the MP as a fusion to green fluorescent protein (GFP). Microscopic analysis of infected protoplasts and of infection sites in leaves of MP-transgenic Nicotiana benthamiana indicates that MP(P81S)-GFP and MP(P81S;T104I;R167K)-GFP differ in subcellular distribution. MP(P81S)-GFP lacks specific sites of accumulation in protoplasts and, in epidermal cells, exclusively localizes to Pd. MP(P81S;T104I;R167K)-GFP, in contrast, in addition localizes to inclusion bodies and microtubules and thus exhibits a subcellular localization pattern that is similar, if not identical, to the pattern reported for wild-type MP-GFP. Since accumulation of MP to inclusion bodies is not required for function, these observations confirm a role for microtubules in TMV RNA cell-to-cell transport.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/11907237-10064612, http://linkedlifedata.com/resource/pubmed/commentcorrection/11907237-10212943, http://linkedlifedata.com/resource/pubmed/commentcorrection/11907237-10380926, http://linkedlifedata.com/resource/pubmed/commentcorrection/11907237-10504577, http://linkedlifedata.com/resource/pubmed/commentcorrection/11907237-10579716, http://linkedlifedata.com/resource/pubmed/commentcorrection/11907237-10753772, http://linkedlifedata.com/resource/pubmed/commentcorrection/11907237-10783235, http://linkedlifedata.com/resource/pubmed/commentcorrection/11907237-10840061, http://linkedlifedata.com/resource/pubmed/commentcorrection/11907237-10849348, http://linkedlifedata.com/resource/pubmed/commentcorrection/11907237-11000113, http://linkedlifedata.com/resource/pubmed/commentcorrection/11907237-11044681, http://linkedlifedata.com/resource/pubmed/commentcorrection/11907237-11050252, http://linkedlifedata.com/resource/pubmed/commentcorrection/11907237-11051555, http://linkedlifedata.com/resource/pubmed/commentcorrection/11907237-11056538, http://linkedlifedata.com/resource/pubmed/commentcorrection/11907237-11070034, http://linkedlifedata.com/resource/pubmed/commentcorrection/11907237-11163140, http://linkedlifedata.com/resource/pubmed/commentcorrection/11907237-11208141, http://linkedlifedata.com/resource/pubmed/commentcorrection/11907237-11215497, http://linkedlifedata.com/resource/pubmed/commentcorrection/11907237-11369897, http://linkedlifedata.com/resource/pubmed/commentcorrection/11907237-11435172, http://linkedlifedata.com/resource/pubmed/commentcorrection/11907237-11452121, http://linkedlifedata.com/resource/pubmed/commentcorrection/11907237-11846035, http://linkedlifedata.com/resource/pubmed/commentcorrection/11907237-1379865, http://linkedlifedata.com/resource/pubmed/commentcorrection/11907237-1392601, http://linkedlifedata.com/resource/pubmed/commentcorrection/11907237-1568243, http://linkedlifedata.com/resource/pubmed/commentcorrection/11907237-1827229, http://linkedlifedata.com/resource/pubmed/commentcorrection/11907237-1984651, http://linkedlifedata.com/resource/pubmed/commentcorrection/11907237-1990065, http://linkedlifedata.com/resource/pubmed/commentcorrection/11907237-2302736, http://linkedlifedata.com/resource/pubmed/commentcorrection/11907237-2466372, http://linkedlifedata.com/resource/pubmed/commentcorrection/11907237-3690662, http://linkedlifedata.com/resource/pubmed/commentcorrection/11907237-5432063, http://linkedlifedata.com/resource/pubmed/commentcorrection/11907237-7851657, http://linkedlifedata.com/resource/pubmed/commentcorrection/11907237-7886938, http://linkedlifedata.com/resource/pubmed/commentcorrection/11907237-8108427, http://linkedlifedata.com/resource/pubmed/commentcorrection/11907237-8124726, http://linkedlifedata.com/resource/pubmed/commentcorrection/11907237-8533085, http://linkedlifedata.com/resource/pubmed/commentcorrection/11907237-8533089, http://linkedlifedata.com/resource/pubmed/commentcorrection/11907237-8615008, http://linkedlifedata.com/resource/pubmed/commentcorrection/11907237-8707060, http://linkedlifedata.com/resource/pubmed/commentcorrection/11907237-8718620, http://linkedlifedata.com/resource/pubmed/commentcorrection/11907237-8718621, http://linkedlifedata.com/resource/pubmed/commentcorrection/11907237-9011088, http://linkedlifedata.com/resource/pubmed/commentcorrection/11907237-9013679, http://linkedlifedata.com/resource/pubmed/commentcorrection/11907237-9126258, http://linkedlifedata.com/resource/pubmed/commentcorrection/11907237-9185584, http://linkedlifedata.com/resource/pubmed/commentcorrection/11907237-9375392, http://linkedlifedata.com/resource/pubmed/commentcorrection/11907237-942051, http://linkedlifedata.com/resource/pubmed/commentcorrection/11907237-9668131, http://linkedlifedata.com/resource/pubmed/commentcorrection/11907237-9681037, http://linkedlifedata.com/resource/pubmed/commentcorrection/11907237-9736708, http://linkedlifedata.com/resource/pubmed/commentcorrection/11907237-9744091, http://linkedlifedata.com/resource/pubmed/commentcorrection/11907237-9789325, http://linkedlifedata.com/resource/pubmed/commentcorrection/11907237-9872750
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0022-538X
pubmed:author
pubmed:issnType
Print
pubmed:volume
76
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
3974-80
pubmed:dateRevised
2010-9-14
pubmed:meshHeading
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